Open Close
Reference
Citation
Wagner, E.J., Burch, B.D., Godfrey, A.C., Salzler, H.R., Duronio, R.J., Marzluff, W.F. (2007). A genome-wide RNA interference screen reveals that variant histones are necessary for replication-dependent histone pre-mRNA processing.  Mol. Cell 28(4): 692--699.
FlyBase ID
FBrf0201575
Publication Type
Research paper
Abstract

Metazoan replication-dependent histone mRNAs are not polyadenylated and instead end in a conserved stem loop that is the cis element responsible for coordinate posttranscriptional regulation of these mRNAs. Using biochemical approaches, only a limited number of factors required for cleavage of histone pre-mRNA have been identified. We therefore performed a genome-wide RNA interference screen in Drosophila cells using a GFP reporter that is expressed only when histone pre-mRNA processing is disrupted. Four of the 24 genes identified encode proteins also necessary for cleavage/polyadenylation, indicating mechanistic conservation in formation of different mRNA 3' ends. We also unexpectedly identified the histone variants H2Av and H3.3A/B. In H2Av mutant cells, U7 snRNP remains active but fails to accumulate at the histone locus, suggesting there is a regulatory pathway that coordinates the production of variant and canonical histones that acts via localization of essential histone pre-mRNA processing factors.

PubMed ID
PubMed Central ID
Related Publication(s)
Supplementary material
Associated Information
Comments
Associated Files
Other Information
Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Mol. Cell
    Title
    Molecular Cell
    Publication Year
    1997-
    ISBN/ISSN
    1097-2765 1097-4164
    Data From Reference
    Alleles (4)
    Genes (34)
    Cell Lines (1)
    Insertions (1)
    Experimental Tools (1)
    Transgenic Constructs (1)