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Neckameyer, W.S., Coleman, C.M., Eadie, S., Goodwin, S.F. (2007). Compartmentalization of neuronal and peripheral serotonin synthesis in Drosophila melanogaster.  Genes Brain Behav. 6(8): 756--769.
FlyBase ID
FBrf0201679
Publication Type
Research paper
Abstract

In Drosophila, one enzyme (Drosophila tryptophan-phenylalanine hydroxylase, DTPHu) hydroxylates both tryptophan to yield 5-hydroxytryptophan, the first step in serotonin synthesis, and phenylalanine, to generate tyrosine. Analysis of the sequenced Drosophila genome identified an additional enzyme with extensive homology to mammalian tryptophan hydroxylase (TPH), which we have termed DTRHn. We have shown that DTRHn can hydroxylate tryptophan in vitro but displays differential activity relative to DTPHu when using tryptophan as a substrate. Recent studies in mice identified the presence of two TPH genes, Tph1 and Tph2, from distinct genetic loci. Tph1 represents the non-neuronal TPH gene, and Tph2 is expressed exclusively in the brain. In this article, we show that DTRHn is neuronal in expression and function and thus represents the Drosophila homologue of Tph2. Using a DTRHn-null mutation, we show that diminished neuronal serotonin affects locomotor, olfactory and feeding behaviors, as well as heart rate. We also show that DTPHu functions in vivo as a phenylalanine hydroxylase in addition to its role as the peripheral TPH in Drosophila, and is critical for non-neuronal developmental events.

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    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Genes Brain Behav.
    Title
    Genes, Brain, and Behavior
    Publication Year
    2002-
    ISBN/ISSN
    1601-1848 1601-183X
    Data From Reference
    Alleles (6)
    Genes (5)
    Insertions (3)
    Experimental Tools (1)
    Transgenic Constructs (4)