Reference Report

Reference
Citation	Haecker, A., Qi, D., Lilja, T., Moussian, B., Andrioli, L.P., Luschnig, S., Mannervik, M. (2007). Drosophila brakeless interacts with atrophin and is required for tailless-mediated transcriptional repression in early embryos. PLoS Biol. 5(6): e145. (Export to RIS)
FlyBase ID	FBrf0201761
Publication Type	Research paper
PubMed ID	17503969
PubMed Abstract	Complex gene expression patterns in animal development are generated by the interplay of transcriptional activators and repressors at cis-regulatory DNA modules (CRMs). How repressors work is not well understood, but often involves interactions with co-repressors. We isolated mutations in the brakeless gene in a screen for maternal factors affecting segmentation of the Drosophila embryo. Brakeless, also known as Scribbler, or Master of thickveins, is a nuclear protein of unknown function. In brakeless embryos, we noted an expanded expression pattern of the Krüppel (Kr) and knirps (kni) genes. We found that Tailless-mediated repression of kni expression is impaired in brakeless mutants. Tailless and Brakeless bind each other in vitro and interact genetically. Brakeless is recruited to the Kr and kni CRMs, and represses transcription when tethered to DNA. This suggests that Brakeless is a novel co-repressor. Orphan nuclear receptors of the Tailless type also interact with Atrophin co-repressors. We show that both Drosophila and human Brakeless and Atrophin interact in vitro, and propose that they act together as a co-repressor complex in many developmental contexts. We discuss the possibility that human Brakeless homologs may influence the toxicity of polyglutamine-expanded Atrophin-1, which causes the human neurodegenerative disease dentatorubral-pallidoluysian atrophy (DRPLA).
DOI	10.1371/journal.pbio.0050145
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Language of Publication	English
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Publication Type	Journal
Abbreviation	PLoS Biol.
Title	PLoS Biology
Publication Year	2003-
ISBN/ISSN	1545-7885 1544-9173
Data from Reference
Aberrations (1)
	Df(2R)PC4
Alleles (17)
	Ecol\lacZ^gt.55x2 Ecol\lacZ^kni.4.4 Ecol\lacZ^{NEEΔsna.Scer\UAS} Ecol\lacZ^{rho.50:50.kni} Ecol\lacZ^{rho.NEEΔsna.180} hb^Scer\FRT.sna hb^sna.PC sbb¹ sbb² sbb¹⁴ sbb²⁷⁸ sbb³³⁹ sbb⁰⁴⁴⁴⁰ sbb^{Kr.T:Scer\GAL4} tll¹ tll^Scer\FRT.sna tll^sna.PH
Constructs (10)
	P{50/50-lacZ} P{gt-lacZ.55x2} P{kni(4.4)-lacZ} P{Kreggy-BksA} P{NEEΔsna.AB} P{sna(FRT.STOP)hb.C} P{sna(FRT.STOP)tll.H} P{sna(-FRT)hb.C} P{sna(-FRT)tll.H} P{UAS-NEE-lacZ}
Genes (11)
	c Ecol\lacZ gt Gug hb kni Kr px sbb T:Scer\GAL4 tll
Natural transposons (1)
	P-element