Reference Report

Reference
Citation	Soltani-Bejnood, M., Thomas, S.E., Villeneuve, L., Schwartz, K., Hong, C., McKee, B.D. (2007). Role of the mod(mdg4) common region in homolog segregation in Drosophila male meiosis. Genetics 176(1): 161--180. (Export to RIS)
FlyBase ID	FBrf0201774
Publication Type	Research paper
PubMed ID	17277376
PubMed Abstract	Homologous chromosomes must pair and establish stable connections during prophase I of meiosis to segregate reliably from each other at anaphase I. In most organisms, the stable connections, called chiasmata, arise from crossovers. In Drosophila males, homologs pair and segregate without crossing over. Chiasmata are replaced by a homolog conjunction complex that includes the Stromalin in Meiosis (SNM) and Modifier of Mdg4 in Meiosis (MNM) proteins. MNM is one of 31 alternative splice products of mod(mdg4), all of which share a common 402-amino-acid N terminus and differ at their C termini. Previous data demonstrated that an MNM-specific exon is required for homolog conjunction, but did not address whether the N-terminal common region, which includes a BTB domain that can mediate coalescence of protein-DNA complexes, is also required. Here we describe a mutation in the common region of mod(mdg4), Z3-3401, that causes qualitatively similar phenotypes as the MNM-specific alleles but disrupts X-Y segregation much more drastically than autosomal segregation. The mutant MNM protein in Z3-3401 is expressed throughout prophase I in spermatocytes but the protein is confined to the cytoplasm, suggesting that the Z3-3401 mutation disrupts a signal required for nuclear localization or retention. Z3-3401 fails to complement a large battery of lethal and semilethal alleles in the common region for meiotic nondisjunction, including an allele containing an amino acid substitution at a conserved residue in the BTB/POZ domain, consistent with a general requirement for the mod(mdg4) common region in homolog segregation.
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Secondary IDs	FBrf0192889
Language of Publication	English
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Parent Publication
Publication Type	Journal
Abbreviation	Genetics
Title	Genetics
Publication Year	1916-
ISBN/ISSN	0016-6731
Data from Reference
Aberrations (2)
	Df(3R)e-Gp4 Df(3R)GC14
Alleles (26)
	Avic\GFP^{Hsp83.T:Ecol\lacI-Δ,T:SV40\nls2} Ecol\lacO^256x mod(mdg4)⁰² mod(mdg4)⁰³ mod(mdg4)²⁰ mod(mdg4)¹¹⁷ mod(mdg4)^142Δ10 mod(mdg4)^142Δ15 mod(mdg4)^142Δ29 mod(mdg4)^142Δ32 mod(mdg4)^142Δ33 mod(mdg4)^142Δ49 mod(mdg4)²⁶⁹ mod(mdg4)³²⁴ mod(mdg4)³⁴⁰ mod(mdg4)^+t7.5 mod(mdg4)^B2 mod(mdg4)^eGp4 mod(mdg4)^{MNM.hs.T:Avic\GFP-EGFP} mod(mdg4)^neo129 mod(mdg4)^T6 mod(mdg4)^T16 mod(mdg4)^Z3-3298 mod(mdg4)^Z3-3401 mod(mdg4)^Z3-5578 y²
Constructs (4)
	P{hs-mod(mdg4).MNM.GFP} P{Hsp83-GFP.lacI} P{lacO.256x} P{mod(mdg4).7.5}
Genes (4)
	Avic\GFP Ecol\lacO mod(mdg4) y
Insertions (4)
	gypsy{}mod(mdg4)⁰² gypsy{}y² P{}mod(mdg4)⁰³ P{hsneo.ry⁺}mod(mdg4)^neo129
Natural transposons (1)
	P-element