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Citation
Norgate, M., Southon, A., Zou, S., Zhan, M., Sun, Y., Batterham, P., Camakaris, J. (2007). Copper homeostasis gene discovery in Drosophila melanogaster.  Biometals 20(3-4): 683--697.
FlyBase ID
FBrf0201775
Publication Type
Research paper
Abstract
Recent studies have shown a high level of conservation between Drosophila melanogaster and mammalian copper homeostasis mechanisms. These studies have also demonstrated the efficiency with which this species can be used to characterize novel genes, at both the cellular and whole organism level. As a versatile and inexpensive model organism, Drosophila is also particularly useful for gene discovery applications and thus has the potential to be extremely useful in identifying novel copper homeostasis genes and putative disease genes. In order to assess the suitability of Drosophila for this purpose, three screening approaches have been investigated. These include an analysis of the global transcriptional response to copper in both adult flies and an embryonic cell line using DNA microarray analysis. Two mutagenesis-based screens were also utilized. Several candidate copper homeostasis genes have been identified through this work. In addition, the results of each screen were carefully analyzed to identify any factors influencing efficiency and sensitivity. These are discussed here with the aim of maximizing the efficiency of future screens and the most suitable approaches are outlined. Building on this information, there is great potential for the further use of Drosophila for copper homeostasis gene discovery.
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PubMed Central ID
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Secondary IDs
  • FBrf0192001
Language of Publication
English
Additional Languages of Abstract
Parent Publication
Publication Type
Journal
Abbreviation
Biometals
Title
Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine
Publication Year
1992-
ISBN/ISSN
0966-0844
Data From Reference
Gene Groups (1)
Genes (20)
Human Disease Models (1)