Reference Report

Reference
Citation	Manak, J.R., Wen, H., Van, T., Andrejka, L., Lipsick, J.S. (2007). Loss of Drosophila Myb interrupts the progression of chromosome condensation. Nat. Cell Biol. 9(5): 581--587. (Export to RIS)
FlyBase ID	FBrf0201787
Publication Type	Research paper
PubMed ID	17450131
PubMed Abstract	Completion of chromosome condensation is required before segregation during the mitotic cell cycle to ensure the transmission of genetic material with high fidelity in a timely fashion. In many eukaryotes this condensation is regulated by phosphorylation of histone H3 on Ser 10 (H3S10). This phosphorylation normally begins in the late-replicating pericentric heterochromatin and then spreads to the earlier replicating euchromatin. Here, we show that these phases of condensation are genetically separable in that the absence of Drosophila Myb causes cells to arrest with H3S10 phosphorylation of heterochromatin but not euchromatin. In addition, we used mosaic analysis to demonstrate that although the Myb protein can be removed in a single cell cycle, the failure of chromosome condensation occurs only after many cell divisions in the absence of Myb protein. The Myb protein is normally located in euchromatic but not heterochromatic regions of the nucleus, suggesting that Myb may be essential for a modification of euchromatin that is required for the efficient spread of chromosome condensation.
DOI	10.1038/ncb1580
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Secondary IDs	FBrf0202279
Language of Publication	English
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Publication Type	Journal
Abbreviation	Nat. Cell Biol.
Title	Nature Cell Biology
Publication Year	1999-
ISBN/ISSN	1465-7392 1476-4679
Data from Reference
Aberrations (1)
	Df(1)MH107
Alleles (11)
	Myb^MH107 Myb^Scer\UAS.cMa Myb^{Scer\UAS.T:Avic\GFP-EGFP} Myb^t9.8 Myb^{T:Avic\GFP-EGFP} Myb^unspecified Scer\GAL4^en-e16E Scer\GAL4^ey.PH Scer\GAL4^T155 Scer\GAL4^unspecified T:Avic\GFP^EGFP
Constructs (6)
	P{GAL4} P{GAL4-ey.H} P{Myb^-,AlkB⁺.9.8} P{Myb-GFP.M} P{UAS-Myb.GFP} P{UAS-Myb.M}
Genes (9)
	AlkB ct CycB His2Av His3 Lam Myb Scer\GAL4 wg
Insertions (2)
	P{en2.4-GAL4}e16E P{GawB}T155
Natural transposons (1)
	P-element