Reference Report

Reference
Citation	Kohsaka, H., Takasu, E., Nose, A. (2007). In vivo induction of postsynaptic molecular assembly by the cell adhesion molecule Fasciclin2. J. Cell Biol. 179(6): 1289--1300. (Export to RIS)
FlyBase ID	FBrf0201824
Publication Type	Research paper
PubMed ID	18070911
PubMed Abstract	Cell adhesion molecules (CAMs) are thought to mediate interactions between innervating axons and their targets. However, such interactions have not been directly observed in vivo. In this paper, we study the function and dynamics of Fasciclin2 (Fas2), a homophilic CAM expressed both pre- and postsynaptically during neuromuscular synapse formation in Drosophila melanogaster. We apply live imaging of functional fluorescent fusion proteins expressed in muscles and find that Fas2 and Discs-Large (Dlg; a scaffolding protein known to bind Fas2) accumulate at the synaptic contact site soon after the arrival of the nerve. Genetic, deletion, and photobleaching analyses suggest that Fas2-mediated trans-synaptic adhesion is important for the postsynaptic accumulation of both Fas2 itself and Dlg. In fas2 mutants, many aspects of synapse formation appear normal; however, we see a reduction in the synaptic accumulation of Scribble (another scaffolding protein) and glutamate receptor subunits GluRIIA and GluRIIB. We propose that Fas2 mediates trans-synaptic adhesion, which contributes to postsynaptic molecular assembly at the onset of synaptogenesis.
DOI	10.1083/jcb.200705154
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Language of Publication	English
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Publication Type	Journal
Abbreviation	J. Cell Biol.
Title	Journal of Cell Biology
Publication Year	1966-
ISBN/ISSN	0021-9525
Data from Reference
Aberrations (2)
	Df(2L)cl-h4 Df(2L)SP22
Alleles (16)
	Avic\GFP^{CFP.Scer\UAS.T:Hsap\Myr2} Avic\GFP^{Scer\UAS.T:Hsap\Myr2} Avic\GFP^{YFP.elav.T:Hsap\Myr2} Avic\GFP^{YFP.Scer\UAS.T:Hsap\Myr2} dlg1⁷ dlg1^{Scer\UAS.T:Avic\GFP-YFP} Fas2^EB112 Fas2^{extra.Scer\UAS.T:Avic\GFP-YFP} Fas2^{intra.Scer\UAS.T:Avic\GFP-YFP} Fas2^{Scer\UAS.T:Avic\GFP-CFP} Fas2^{Scer\UAS.T:Avic\GFP-YFP} pros^m4 Scer\GAL4^elav.PLu Scer\GAL4^tey-5053A T:Avic\GFP^CFP T:Avic\GFP^YFP
Constructs (10)
	P{elav-mYFP.K} P{GAL4-elav.L} P{UAS-extra-Fas2.YFP} P{UAS-Fas2.CFP} P{UAS-Fas2.YFP} P{UAS-gapGFP} P{UAS-intra-Fas2.YFP} P{UAS-mCFP.K} P{UAS-mYFP.K} P{UAS-YFP-dlg1.K}
Genes (22)
	Ank Avic\GFP baz brp cora dlg1 Dys Fas2 GluRIIA GluRIIB Grip gukh if mew Pak pros Scer\GAL4 scrib T:Hsap\Myr2 veli α-Spec β-Spec
Insertions (1)
	P{GawB}tey^5053A
Natural transposons (1)
	P-element