Reference Report

Reference
Citation	Jakobsen, J.S., Braun, M., Astorga, J., Gustafson, E.H., Sandmann, T., Karzynski, M., Carlsson, P., Furlong, E.E.M. (2007). Temporal ChIP-on-chip reveals Biniou as a universal regulator of the visceral muscle transcriptional network. Genes Dev. 21(19): 2448--2460. (Export to RIS)
FlyBase ID	FBrf0201841
Publication Type	Research paper
PubMed ID	17908931
PubMed Abstract	Smooth muscle plays a prominent role in many fundamental processes and diseases, yet our understanding of the transcriptional network regulating its development is very limited. The FoxF transcription factors are essential for visceral smooth muscle development in diverse species, although their direct regulatory role remains elusive. We present a transcriptional map of Biniou (a FoxF transcription factor) and Bagpipe (an Nkx factor) activity, as a first step to deciphering the developmental program regulating Drosophila visceral muscle development. A time course of chromatin immunoprecipitatation followed by microarray analysis (ChIP-on-chip) experiments and expression profiling of mutant embryos reveal a dynamic map of in vivo bound enhancers and direct target genes. While Biniou is broadly expressed, it regulates enhancers driving temporally and spatially restricted expression. In vivo reporter assays indicate that the timing of Biniou binding is a key trigger for the time span of enhancer activity. Although bagpipe and biniou mutants phenocopy each other, their regulatory potential is quite different. This network architecture was not apparent from genetic studies, and highlights Biniou as a universal regulator in all visceral muscle, regardless of its developmental origin or subsequent function. The regulatory connection of a number of Biniou target genes is conserved in mice, suggesting an ancient wiring of this developmental program.
DOI	10.1101/gad.437607
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Language of Publication	English
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Parent Publication
Publication Type	Journal
Abbreviation	Genes Dev.
Title	Genes & Development
Publication Year	1987-
ISBN/ISSN	0890-9369
Data from Reference
Aberrations (2)
	Df(3R)e-D7 Df(3R)e-F1
Alleles (17)
	Avic\GFP^{2L.3813750-3815714.T:nls-tra} Avic\GFP^{bap.FH-VME.T:nls-tra} Avic\GFP^{CG8503.VME.T:nls-tra} Avic\GFP^{dac.VME.T:nls-tra} Avic\GFP^{fd64A.eVME.T:nls-tra} Avic\GFP^{fd64A.lVME.T:nls-tra} Avic\GFP^{HLH54F.VME.T:nls-tra} Avic\GFP^{hs.Stinger.T:nls-tra} Avic\GFP^{ken.VME.T:nls-tra} Avic\GFP^{lmd.VME.T:nls-tra} Avic\GFP^{mib2.VME.T:nls-tra} Avic\GFP^{Neurochondrin.VME.T:nls-tra} Avic\GFP^{otk.VME.T:nls-tra} Avic\GFP^{slp1.VME.T:nls-tra} Avic\GFP^{ttk.VME.T:nls-tra} bin^R22 tin^+t10.7
Constructs (15)
	P{2L.3813750-3815714-GFP} P{bap-GFP.FH-VME} P{CaSperRe28} P{CG8503-GFP.VME} P{dac-GFP.VME} P{fd64A-GFP.eVME} P{fd64A-GFP.VME} P{HLH54F-GFP.VME} P{ken-GFP.VME} P{lmd-GFP.VME} P{mib2-GFP.VME} P{Neurochondrin-GFP.VME} P{otk-GFP.VME} P{slp1-GFP.VME} P{ttk-GFP.VME}
Genes (22)
	Avic\GFP bap bin bnl CG4580 CG17181 Con dac dpp Fas3 Hand HLH54F lmd Neurochondrin pnt salm slp1 sns T:nls-tra tin vimar βTub60D
Natural transposons (1)
	P-element
Sequence features (13)
	bap_FHVME CG8503_VME dac_VME fd64a_eVME fd64A_VME HLH54F_VME ken_VME lmd_VME mib2_VME Neurochondrin_VME otk_VME slp1_VME ttk_VME