Reference Report

Reference
Citation	Dahlgaard, K., Raposo, A.A., Niccoli, T., St Johnston, D. (2007). Capu and Spire assemble a cytoplasmic actin mesh that maintains microtubule organization in the Drosophila Oocyte. Dev. Cell 13(4): 539--553. (Export to RIS)
FlyBase ID	FBrf0201859
Publication Type	Research paper
PubMed ID	17925229
PubMed Abstract	Mutants in the actin nucleators Cappuccino and Spire disrupt the polarized microtubule network in the Drosophila oocyte that defines the anterior-posterior axis, suggesting that microtubule organization depends on actin. Here, we show that Cappuccino and Spire organize an isotropic mesh of actin filaments in the oocyte cytoplasm. capu and spire mutants lack this mesh, whereas overexpressed truncated Cappuccino stabilizes the mesh in the presence of Latrunculin A and partially rescues spire mutants. Spire overexpression cannot rescue capu mutants, but prevents actin mesh disassembly at stage 10B and blocks late cytoplasmic streaming. We also show that the actin mesh regulates microtubules indirectly, by inhibiting kinesin-dependent cytoplasmic flows. Thus, the Capu pathway controls alternative states of the oocyte cytoplasm: when active, it assembles an actin mesh that suppresses kinesin motility to maintain a polarized microtubule cytoskeleton. When inactive, unrestrained kinesin movement generates flows that wash microtubules to the cortex.
DOI	10.1016/j.devcel.2007.09.003
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Dev. Cell
Title	Developmental Cell
Publication Year	2001-
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Data from Reference
Aberrations (2)
	Df(2L)ed1 Df(2L)Exel6046
Alleles (16)
	capu¹ capu² capu^2F capu^{Scer\UAS.P\T.cDa.T:Avic\GFP} capu^{Scer\UAS.P\T.cDa} capu^{ΔN.Scer\UAS.P\T.T:Avic\GFP} chic²²¹ chic⁰¹³²⁰ Khc¹⁷ Khc²⁷ par-1^{N1S.linker.Scer\UAS.T:Avic\GFP-m6} Scer\GAL4^{nos.UTR.T:Hsim\VP16} spir¹ spir^2F spir^{RC.Scer\UAS.P\T.T:Avic\GFP} spir^{RD.Scer\UAS.P\T.T:Avic\GFP}
Constructs (7)
	P{GAL4::VP16-nos.UTR} P{UAS-par-1.N1S.linker-mGFP6} P{UASp-capu.D} P{UASp-capu.GFP.D} P{UASp-capu.GFP.ΔN} P{UASp-spir.GFP.RD} P{UASp-spir.RC.GFP}
Genes (8)
	capu chic Khc osk par-1 Scer\GAL4 spir T:Avic\GFP
Natural transposons (1)
	P-element