Reference Report

Reference
Citation	Parma, D.H., Bennett, P.E., Boswell, R.E. (2007). Mago Nashi and Tsunagi/Y14, respectively, regulate Drosophila germline stem cell differentiation and oocyte specification. Dev. Biol. 308(2): 507--519. (Export to RIS)
FlyBase ID	FBrf0201988
Publication Type	Research paper
PubMed ID	17628520
PubMed Abstract	A protein complex consisting of Mago Nashi and Tsunagi/Y14 is required to establish the major body axes and for the localization of primordial germ cell determinants during Drosophila melanogaster oogenesis. The Mago Nashi:Tsunagi/Y14 heterodimer also serves as the core of the exon junction complex (EJC), a multiprotein complex assembled on spliced mRNAs. In previous studies, reduced function alleles of mago nashi and tsunagi/Y14 were used to characterize the roles of the genes in oogenesis. Here, we investigated mago nashi and tsunagi/Y14 using null alleles and clonal analysis. Germline clones lacking mago nashi function divide but fail to differentiate. The mago nashi null germline stem cells produce clones over a period of at least 11 days, suggesting that mago nashi is not necessary for stem cell self-renewal. However, germline stem cells lacking tsunagi/Y14 function are indistinguishable from wild type. Additionally, in tsunagi/Y14 null germline cysts, centrosomes and oocyte-specific components fail to concentrate within a single cell and oocyte fate is not restricted to a single cell. Together, our results suggest not only that mago nashi is required for germline stem cell differentiation but that surprisingly mago nashi functions independently of tsunagi/Y14 in this process. On the other hand, Tsunagi/Y14 is essential for restricting oocyte fate to a single cell and may function with mago nashi in this process.
DOI	10.1016/j.ydbio.2007.06.007
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Dev. Biol.
Title	Developmental Biology
Publication Year	1959-
ISBN/ISSN	0012-1606
Data from Reference
Alleles (8)
	Avic\GFP^bam.-799+133 bgcn¹ His2Av^{T:Avic\GFP-S65T} mago^SHL1 mago^T:Avic\GFP mago^T:Hsap\MYC Scer\GAL4^C587 tsu^Δ3-5
Constructs (4)
	P{bam-GFP.-799/+133} P{GFP-mago} P{His2Av^{T:Avic\GFP-S65T}} P{myc-mago}
Genes (14)
	Avic\GFP bam bgcn c(3)G cp309 egl His2Av mago orb Scer\GAL4 tj tsu vas α-Spec
Insertions (2)
	P{EP}tsu^EP567 P{GAL4}C587