Reference Report

Reference
Citation	Zhang, J., Megraw, T.L. (2007). Proper recruitment of gamma-tubulin and D-TACC/Msps to embryonic Drosophila centrosomes requires Centrosomin Motif 1. Mol. Biol. Cell 18(10): 4037--4049. (Export to RIS)
FlyBase ID	FBrf0202160
Publication Type	Research paper
PubMed ID	17671162
PubMed Abstract	Centrosomes are microtubule-organizing centers and play a dominant role in assembly of the microtubule spindle apparatus at mitosis. Although the individual binding steps in centrosome maturation are largely unknown, Centrosomin (Cnn) is an essential mitotic centrosome component required for assembly of all other known pericentriolar matrix (PCM) proteins to achieve microtubule-organizing activity at mitosis in Drosophila. We have identified a conserved motif (Motif 1) near the amino terminus of Cnn that is essential for its function in vivo. Cnn Motif 1 is necessary for proper recruitment of gamma-tubulin, D-TACC (the homolog of vertebrate transforming acidic coiled-coil proteins [TACC]), and Minispindles (Msps) to embryonic centrosomes but is not required for assembly of other centrosome components including Aurora A kinase and CP60. Centrosome separation and centrosomal satellite formation are severely disrupted in Cnn Motif 1 mutant embryos. However, actin organization into pseudocleavage furrows, though aberrant, remains partially intact. These data show that Motif 1 is necessary for some but not all of the activities conferred on centrosome function by intact Cnn.
DOI	10.1091/mbc.E07-05-0474
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Mol. Biol. Cell
Title	Molecular Biology of the Cell
Publication Year	1992-
ISBN/ISSN	1059-1524
Data from Reference
Aberrations (1)
	Df(2R)cnn^25cn1
Alleles (9)
	CG42321^25cn1 cnn^25cn1 cnn^HK21 cnn^Scim cnn^{WT.Scer\UAS.T:Avic\GFP-EGFP} cnn^{Δ1.Scer\UAS.T:Avic\GFP-EGFP} Scer\GAL4^{nos.UTR.T:Hsim\VP16} Scer\GAL4^tub.PU T:Avic\GFP^EGFP
Constructs (4)
	P{GAL4::VP16-nos.UTR} P{tub-GAL4.U} P{UASp-cnn.WT} P{UASp-cnn.Δ1}
Genes (11)
	aur CG42321 cnn Grip84 Map60 msps Nek2 Scer\GAL4 tacc γTub23C γTub37C
Insertions (1)
	P{SUPor-P}cnn^Scim
Natural transposons (1)
	P-element