Reference Report

Reference
Citation	Minidorff, E.N., O'Keefe, D.D., Labbe, A., Yang, J.P., Ou, Y., Yoshikawa, S., van Meyel, D.J. (2007). A Gain-of-Function Screen for Genes That Influence Axon Guidance Identifies the NF-κB Protein Dorsal and Reveals a Requirement for the Kinase Pelle in Drosophila Photoreceptor Axon Targeting. Genetics 176(4): 2247--2263. (Export to RIS)
FlyBase ID	FBrf0202193
Publication Type	Research paper
PubMed ID	17603113
PubMed Abstract	To identify novel regulators of nervous system development, we used the GAL4-UAS misexpression system in Drosophila to screen for genes that influence axon guidance in developing embryos. We mobilized the Gene Search (GS) P element and identified 42 lines with insertions in unique loci, including leak/roundabout2, which encodes an axon guidance receptor and confirms the utility of our screen. The genes we identified encode proteins of diverse classes, some acting near the cell surface and others in the cytoplasm or nucleus. We found that one GS line drove misexpression of the NF-kappaB transcription factor Dorsal, causing motor axons to bypass their correct termination sites. In the developing visual system, Dorsal misexpression also caused photoreceptor axons to reach incorrect positions within the optic lobe. This mistargeting occurred without observable changes of cell fate and correlated with localization of ectopic Dorsal in distal axons. We found that Dorsal and its inhibitor Cactus are expressed in photoreceptors, though neither was required for axon targeting. However, mutation analyses of genes known to act upstream of Dorsal revealed a requirement for the interleukin receptor-associated kinase family kinase Pelle for layer-specific targeting of photoreceptor axons, validating our screen as a means to identify new molecular determinants of nervous system development in vivo.
DOI	10.1534/genetics.107.072819
Related Publication(s)
Supplementary material	Supplemental Table. [FBrf0206126]
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Secondary IDs
Language of Publication	English
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Also Published As
Parent Publication
Publication Type	Journal
Abbreviation	Genetics
Title	Genetics
Publication Year	1916-
ISBN/ISSN	0016-6731
Data from Reference
Aberrations (6)
	Df(2L)cact-255rv64 Df(2L)H20 Df(2L)J4 Df(2L)r10 Df(2L)TW119 Df(3R)XM3
Alleles (84)
	cact¹ cact⁴ Dif¹ Dif² dl¹ dl⁴ dl^{B.Scer\UAS.T:Ivir\HA1} dl^C447 dl^D447 dl^GSd447 dl^Scer\UAS.cMa Ecol\lacZ^{ro.T:Btau\MAPT} GSd031^GSd031 GSd033^GSd033 GSd034^GSd034 GSd057^GSd057 GSd079^GSd079 GSd201^GSd201 GSd204^GSd204 GSd209^GSd209 GSd210^GSd210 GSd211^GSd211 GSd212^GSd212 GSd214^GSd214 GSd217^GSd217 GSd219^GSd219 GSd220^GSd220 GSd226^GSd226 GSd227^GSd227 GSd229^GSd229 GSd231^GSd231 GSd233^GSd233 GSd236^GSd236 GSd243^GSd243 GSd246^GSd246 GSd247^GSd247 GSd248^GSd248 GSd302^GSd302 GSd312^GSd312 GSd315^GSd315 GSd317^GSd317 GSd318^GSd318 GSd319^GSd319 GSd325^GSd325 GSd328^GSd328 GSd329^GSd329 GSd402^GSd402 GSd404^GSd404 GSd405^GSd405 GSd406^GSd406 GSd411^GSd411 GSd417^GSd417 GSd420^GSd420 GSd427^GSd427 GSd428^GSd428 GSd431^GSd431 GSd433^GSd433 GSd434^GSd434 GSd439^GSd439 GSd446^GSd446 GSd450^GSd450 GSd458^GSd458 GSd462^GSd462 GSd464^GSd464 GSd468^GSd468 GSd469^GSd469 GSd481^GSd481 GSd482^GSd482 GSd486^GSd486 GSd489^GSd489 GSd496^GSd496 GSd497^GSd497 pll² pll⁷ pll²⁵ Scer\GAL4^GMR.PF Scer\GAL4^lz-gal4 Scer\GAL4^MT14 Scer\GAL4^sca-109-68 Scer\GAL4^scrt-11-6 Tl⁴ Tl^rK344 tub² w^+mC
Constructs (3)
	P{ro.lacZ-tau.G} P{UAS-dl.B-HA} P{UASp-dl.M}
Genes (126)
	Akap200 apt babos bl bnl boss Btk29A cact cbt CG2277 CG2469 CG2617 CG5050 CG7518 CG9582 CG30389 CG42674 chp Dif dimm dl Dp1 Ecol\lacZ esg faf Fas2 for foxo GSd031 GSd033 GSd034 GSd057 GSd079 GSd201 GSd204 GSd209 GSd210 GSd211 GSd212 GSd214 GSd217 GSd219 GSd220 GSd226 GSd227 GSd229 GSd231 GSd233 GSd236 GSd243 GSd246 GSd247 GSd248 GSd302 GSd312 GSd315 GSd317 GSd318 GSd319 GSd325 GSd328 GSd329 GSd402 GSd404 GSd405 GSd406 GSd411 GSd417 GSd420 GSd427 GSd428 GSd431 GSd433 GSd434 GSd439 GSd446 GSd450 GSd458 GSd462 GSd464 GSd468 GSd469 GSd481 GSd482 GSd486 GSd489 GSd496 GSd497 GstE1 H hdc Hsrω IM1 imd ken Kr-h1 lea lig mam mir-313 mirr nos otk pll pnt pros psq rdo Rel repo Rho1 run sbb Scer\GAL4 slam spen Stat92E T:Ivir\HA1 tara Tl Trl ttk tub w woc Xrp1
Insertions (57)
	P{GawB}lz^gal4 P{GawB}MT14 P{GawB}scrt^11-6 P{GSV1}dl^GSd447 P{GSV1}GSd031^GSd031 P{GSV1}GSd033^GSd033 P{GSV1}GSd034^GSd034 P{GSV1}GSd057^GSd057 P{GSV1}GSd079^GSd079 P{GSV1}GSd201^GSd201 P{GSV1}GSd204^GSd204 P{GSV1}GSd209^GSd209 P{GSV1}GSd210^GSd210 P{GSV1}GSd211^GSd211 P{GSV1}GSd212^GSd212 P{GSV1}GSd214^GSd214 P{GSV1}GSd217^GSd217 P{GSV1}GSd219^GSd219 P{GSV1}GSd220^GSd220 P{GSV1}GSd226^GSd226 P{GSV1}GSd227^GSd227 P{GSV1}GSd229^GSd229 P{GSV1}GSd231^GSd231 P{GSV1}GSd233^GSd233 P{GSV1}GSd236^GSd236 P{GSV1}GSd243^GSd243 P{GSV1}GSd247^GSd247 P{GSV1}GSd248^GSd248 P{GSV1}GSd302^GSd302 P{GSV1}GSd312^GSd312 P{GSV1}GSd315^GSd315 P{GSV1}GSd317^GSd317 P{GSV1}GSd318^GSd318 P{GSV1}GSd325^GSd325 P{GSV1}GSd328^GSd328 P{GSV1}GSd329^GSd329 P{GSV1}GSd402^GSd402 P{GSV1}GSd404^GSd404 P{GSV1}GSd405^GSd405 P{GSV1}GSd411^GSd411 P{GSV1}GSd417^GSd417 P{GSV1}GSd420^GSd420 P{GSV1}GSd427^GSd427 P{GSV1}GSd428^GSd428 P{GSV1}GSd431^GSd431 P{GSV1}GSd433^GSd433 P{GSV1}GSd434^GSd434 P{GSV1}GSd446^GSd446 P{GSV1}GSd450^GSd450 P{GSV1}GSd458^GSd458 P{GSV1}GSd468^GSd468 P{GSV1}GSd469^GSd469 P{GSV1}GSd482^GSd482 P{GSV1}GSd486^GSd486 P{GSV1}GSd489^GSd489 P{GSV1}GSd496^GSd496 P{GSV1}GSd497^GSd497
Natural transposons (1)
	P-element