Reference Report

Reference
Citation	Johansson, A.M., Stenberg, P., Bernhardsson, C., Larsson, J. (2007). Painting of fourth and chromosome-wide regulation of the 4th chromosome in Drosophila melanogaster. EMBO J. 26(9): 2307--2316. (Export to RIS)
FlyBase ID	FBrf0202200
Publication Type	Research paper
PubMed ID	17318176
PubMed Abstract	Drosophila melanogaster exhibits two expression-regulating systems that target whole, specific chromosomes: the dosage compensation system whereby the male-specific lethal complex doubles transcription of genes on the male X-chromosome and the chromosome 4-specific protein Painting of fourth, POF. POF is the first example of an autosome-specific protein and its presence raises the question of the universality of chromosome-specific regulation. Here we show that POF and heterochromatin protein 1 (HP1) are involved in the global regulation of the 4th chromosome. Contrary to previous conclusions, Pof is not essential for survival of diplo-4th karyotype flies. However, Pof is essential for survival of haplo-4th individuals and expression of chromosome 4 genes in diplo-4th individuals is decreased in the absence of Pof. Mapping of POF using chromatin immunoprecipitation suggested that it binds within genes. Furthermore, we show that POF binding is dependent on heterochromatin and that POF and HP1 bind interdependently to the 4th chromosome. We propose a balancing mechanism involving POF and HP1 that provides a feedback system for fine-tuning expression status of genes on the 4th chromosome.
DOI
Related Publication(s)
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Associated Information
Comments
Associated Files
Other Information
Secondary IDs	FBrf0195036
Language of Publication	English
Additional Languages of Abstract
Also Published As
Parent Publication
Publication Type	Journal
Abbreviation	EMBO J.
Title	The EMBO Journal
Publication Year	1982-
ISBN/ISSN	0261-4189
Data from Reference
Aberrations (5)
	Df(3R)D2 Df(3R)D31A Df(3R)Pof-D140 T(1;4)w^m5 T(1;4)w^m258-18
Alleles (29)
	CG33228^+tLa CG33228^CB-6312-3 CG33228^D2 CG33228^D31A CG33228^SH0542 His2Av⁸¹⁰ Mmp1^D140 Pof^+tLa Pof^D2 Pof^D31A Pof^D114 Pof^D119 Pof^D134 Pof^D139 Pof^D146 Pof^D152 Pof^D154 Pof^D162 Pof^D163 Pof^D191 Pof^D213 Pof^D244 Su(var)3-7^R2a8 Su(var)3-9⁶ Su(var)3-9^evo Su(var)205⁴ Su(var)205⁵ Su(var)205^{dsRNA.Sym.Scer\UAS} SuUR^unspecified
Constructs (2)
	P{Pof^+tLa} P{Sym-UAS-Su(var)205}
Genes (20)
	CaMKII CG1732 CG4806 CG11076 CG31998 CG33228 gw His2Av MED26 Mmp1 PMCA Pof Pur-α Spt-I Su(var)3-7 Su(var)3-9 Su(var)205 SuUR Xbp1 Zyx
Insertions (3)
	P{EP}Pof^EP2285 P{lacW}CG33228^SH0542 P{RS3}CG33228^CB-6312-3
Natural transposons (1)
	P-element