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Citation
Picot, M., Cusumano, P., Klarsfeld, A., Ueda, R., Rouyer, F. (2007). Light activates output from evening neurons and inhibits output from morning neurons in the Drosophila circadian clock.  PLoS Biol. 5(11): e315.
FlyBase ID
FBrf0202589
Publication Type
Research paper
Abstract

Animal circadian clocks are based on multiple oscillators whose interactions allow the daily control of complex behaviors. The Drosophila brain contains a circadian clock that controls rest-activity rhythms and relies upon different groups of PERIOD (PER)-expressing neurons. Two distinct oscillators have been functionally characterized under light-dark cycles. Lateral neurons (LNs) that express the pigment-dispersing factor (PDF) drive morning activity, whereas PDF-negative LNs are required for the evening activity. In constant darkness, several lines of evidence indicate that the LN morning oscillator (LN-MO) drives the activity rhythms, whereas the LN evening oscillator (LN-EO) does not. Since mutants devoid of functional CRYPTOCHROME (CRY), as opposed to wild-type flies, are rhythmic in constant light, we analyzed transgenic flies expressing PER or CRY in the LN-MO or LN-EO. We show that, under constant light conditions and reduced CRY function, the LN evening oscillator drives robust activity rhythms, whereas the LN morning oscillator does not. Remarkably, light acts by inhibiting the LN-MO behavioral output and activating the LN-EO behavioral output. Finally, we show that PDF signaling is not required for robust activity rhythms in constant light as opposed to its requirement in constant darkness, further supporting the minor contribution of the morning cells to the behavior in the presence of light. We therefore propose that day-night cycles alternatively activate behavioral outputs of the Drosophila evening and morning lateral neurons.

PubMed ID
PubMed Central ID
PMC2229858 (PMC) (EuropePMC)
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Secondary IDs
  • FBrf0202300
Language of Publication
English
Additional Languages of Abstract
Parent Publication
Publication Type
Journal
Abbreviation
PLoS Biol.
Title
PLoS Biology
Publication Year
2003-
ISBN/ISSN
1545-7885 1544-9173
Data From Reference
Alleles (12)
Genes (7)
Natural transposons (1)
Insertions (3)
Experimental Tools (1)
Transgenic Constructs (8)