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Shcherbata, H.R., Ward, E.J., Fischer, K.A., Yu, J.Y., Reynolds, S.H., Chen, C.H., Xu, P., Hay, B.A., Ruohola-Baker, H. (2007). Stage-specific differences in the requirements for germline stem cell maintenance in the Drosophila ovary.  Cell Stem Cell 1(6): 698--709.
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Research paper

In this study, we uncover a role for microRNAs in Drosophila germline stem cell (GSC) maintenance. Disruption of Dicer-1 function in GSCs during adult life results in GSC loss. Surprisingly, however, loss of Dicer-1 during development does not result in a GSC maintenance defect, although a defect is seen if both Dicer-1 and Dicer-2 function are disrupted. Loss of the bantam microRNA mimics the Dicer-1 maintenance defect when induced in adult GSCs, suggesting that bantam plays a key role in GSC self-renewal. Mad, a component of the TGF-beta pathway, behaves similarly to Dicer-1: adult GSC maintenance requires Mad if it is lost during adult life, but not if it is lost during pupal development. Overall, these results show stage-specific differential sensitivity of GSC maintenance to certain perturbations, and suggest that there may be Dcr-2 dependent redundancy of GSC maintenance mechanisms during development that is lost in later life.

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PubMed Central ID
PMC2211735 (PMC) (EuropePMC)
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    Publication Type
    Cell Stem Cell
    Cell Stem Cell
    Publication Year
    1934-5909 1875-9777
    Data From Reference
    Aberrations (1)
    Alleles (15)
    Genes (14)
    Natural transposons (1)
    Insertions (3)
    Experimental Tools (2)
    Transgenic Constructs (6)