Reference Report

Reference
Citation	Geisbrecht, E.R., Haralalka, S., Swanson, S.K., Florens, L., Washburn, M.P., Abmayr, S.M. (2008). Drosophila ELMO/CED-12 interacts with Myoblast city to direct myoblast fusion and ommatidial organization. Dev. Biol. 314(1): 137--149. (Export to RIS)
FlyBase ID	FBrf0202723
Publication Type	Research paper
PubMed ID	18163987
PubMed Abstract	Members of the CDM (CED-5, Dock180, Myoblast city) superfamily of guanine nucleotide exchange factors function in diverse processes that include cell migration and myoblast fusion. Previous studies have shown that the SH3, DHR1 and DHR2 domains of Myoblast city (MBC) are essential for it to direct myoblast fusion in the Drosophila embryo, while the conserved DCrk-binding proline rich region is expendable. Herein, we describe the isolation of Drosophila ELMO/CED-12, an approximately 82 kDa protein with a pleckstrin homology (PH) and proline-rich domain, by interaction with the MBC SH3 domain. Mass spectrometry confirms the presence of an MBC/ELMO complex within the embryonic musculature at the time of myoblast fusion and embryos maternally and/or zygotically mutant for elmo exhibit defects in myoblast fusion. Overexpression of MBC and ELMO in the embryonic mesoderm causes defects in myoblast fusion reminiscent of those seen with constitutively-activated Rac1, supporting the previous finding that both the absence of and an excess of Rac activity are deleterious to myoblast fusion. Overexpression of MBC and ELMO/CED-12 in the eye causes perturbations in ommatidial organization that are suppressed by mutations in Rac1 and Rac2, demonstrating genetically that MBC and ELMO/CED-12 cooperate to activate these small GTPases in Drosophila.
DOI	10.1016/j.ydbio.2007.11.022
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Dev. Biol.
Title	Developmental Biology
Publication Year	1959-
ISBN/ISSN	0012-1606
Data from Reference
Aberrations (2)
	Df(2L)HO55 Df(2L)Prl
Alleles (21)
	Ced-12^8C6 Ced-12^9F4 Ced-12^19F3 Ced-12^c06760 Ced-12^{dsRNA.Scer\UAS} Ced-12^Scer\UAS.cGa Ced-12^{Scer\UAS.T:Ivir\HA1} Crk^{dsRNA.Scer\UAS} mbc^D11.2 mbc^{dsRNA.Scer\UAS} mbc^Scer\UAS.cBa mbc^{Scer\UAS.T:Ivir\HA1} Mmus\Cd8a^{Scer\UAS.T:Avic\GFP} Rac1^J11 Rac1^N17.Scer\UAS Rac2^Δ Scer\GAL4^Act.PU Scer\GAL4^c306 Scer\GAL4^GMR.PU Scer\GAL4^Mef2.PR Scer\GAL4^slbo.2.6
Constructs (13)
	P{Act-GAL4.U} P{GAL4-Mef2.R} P{GAL4-slbo.2.6} P{GMR-GAL4.U} P{UAS-Ced-12.G} P{UAS-Ced-12.HA} P{UAS-Crk.IR} P{UAS-ELMO.IR} P{UAS-mbc.B} P{UAS-mbc.HA} P{UAS-mbc.IR} P{UAS-mCD8::GFP.L} P{UAS-Rac1.N17}
Genes (12)
	Ced-12 Crk Kr mbc Mef2 Mhc Mmus\Cd8a Rac1 Rac2 Scer\GAL4 sns T:Ivir\HA1
Insertions (3)
	P{GawB}c306 P{lacW}Aats-thr^k04203 PBac{PB}Ced-12^c06760