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Citation
Acharya, J.K., Dasgupta, U., Rawat, S.S., Yuan, C., Sanxaridis, P.D., Yonamine, I., Karim, P., Nagashima, K., Brodsky, M.H., Tsunoda, S., Acharya, U. (2008). Cell-nonautonomous function of ceramidase in photoreceptor homeostasis.  Neuron 57(1): 69--79.
FlyBase ID
FBrf0202730
Publication Type
Research paper
Abstract

Neutral ceramidase, a key enzyme of sphingolipid metabolism, hydrolyzes ceramide to sphingosine. These sphingolipids are critical structural components of cell membranes and act as second messengers in diverse signal transduction cascades. Here, we have isolated and characterized functional null mutants of Drosophila ceramidase. We show that secreted ceramidase functions in a cell-nonautonomous manner to maintain photoreceptor homeostasis. In the absence of ceramidase, photoreceptors degenerate in a light-dependent manner, are defective in normal endocytic turnover of rhodopsin, and do not respond to light stimulus. Consistent with a cell-nonautonomous function, overexpression of ceramidase in tissues distant from photoreceptors suppresses photoreceptor degeneration in an arrestin mutant and facilitates membrane turnover in a rhodopsin null mutant. Furthermore, our results show that secreted ceramidase is internalized and localizes to endosomes. Our findings establish a role for a secreted sphingolipid enzyme in the regulation of photoreceptor structure and function.

PubMed ID
PubMed Central ID
PMC2271043 (PMC) (EuropePMC)
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    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Neuron
    Title
    Neuron
    Publication Year
    1988-
    ISBN/ISSN
    0896-6273
    Data From Reference
    Alleles (7)
    Genes (10)
    Physical Interactions (1)
    Natural transposons (1)
    Transgenic Constructs (3)