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Citation
Bejarano, F., Luque, C.M., Herranz, H., Sorrosal, G., Rafel, N., Pham, T.T., Milán, M. (2008). A gain-of-function suppressor screen for genes involved in dorsal-ventral boundary formation in the Drosophila wing.  Genetics 178(1): 307--323.
FlyBase ID
FBrf0202749
Publication Type
Research paper
Abstract

The Drosophila wing primordium is subdivided into a dorsal (D) and a ventral (V) compartment by the activity of the LIM-homeodomain protein Apterous in D cells. Cell interactions between D and V cells induce the activation of Notch at the DV boundary. Notch is required for the maintenance of the compartment boundary and the growth of the wing primordium. Beadex, a gain-of-function allele of dLMO, results in increased levels of dLMO protein, which interferes with the activity of Apterous and results in defects in DV axis formation. We performed a gain-of-function enhancer-promoter (EP) screen to search for suppressors of Beadex when overexpressed in D cells. We identified 53 lines corresponding to 35 genes. Loci encoding for micro-RNAs and proteins involved in chromatin organization, transcriptional control, and vesicle trafficking were characterized in the context of dLMO activity and DV boundary formation. Our results indicate that a gain-of-function genetic screen in a sensitized background, as opposed to classical loss-of-function-based screenings, is a very efficient way to identify redundant genes involved in a developmental process.

PubMed ID
PubMed Central ID
PMC2206080 (PMC) (EuropePMC)
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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Genetics
    Title
    Genetics
    Publication Year
    1916-
    ISBN/ISSN
    0016-6731
    Data From Reference
    Aberrations (17)
    Alleles (106)
    Genes (59)
    Natural transposons (1)
    Insertions (62)
    Experimental Tools (3)
    Transgenic Constructs (11)