Reference Report

Reference
Citation	Bejarano, F., Luque, C.M., Herranz, H., Sorrosal, G., Rafel, N., Pham, T.T., Milán, M. (2008). A gain-of-function suppressor screen for genes involved in dorsal-ventral boundary formation in the Drosophila wing. Genetics 178(1): 307--323. (Export to RIS)
FlyBase ID	FBrf0202749
Publication Type	Research paper
PubMed ID	18202376
PubMed Abstract	The Drosophila wing primordium is subdivided into a dorsal (D) and a ventral (V) compartment by the activity of the LIM-homeodomain protein Apterous in D cells. Cell interactions between D and V cells induce the activation of Notch at the DV boundary. Notch is required for the maintenance of the compartment boundary and the growth of the wing primordium. Beadex, a gain-of-function allele of dLMO, results in increased levels of dLMO protein, which interferes with the activity of Apterous and results in defects in DV axis formation. We performed a gain-of-function enhancer-promoter (EP) screen to search for suppressors of Beadex when overexpressed in D cells. We identified 53 lines corresponding to 35 genes. Loci encoding for micro-RNAs and proteins involved in chromatin organization, transcriptional control, and vesicle trafficking were characterized in the context of dLMO activity and DV boundary formation. Our results indicate that a gain-of-function genetic screen in a sensitized background, as opposed to classical loss-of-function-based screenings, is a very efficient way to identify redundant genes involved in a developmental process.
DOI	10.1534/genetics.107.081869
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Secondary IDs
Language of Publication	English
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Publication Type	Journal
Abbreviation	Genetics
Title	Genetics
Publication Year	1916-
ISBN/ISSN	0016-6731
Data from Reference
Aberrations (17)
	Df(2L)C144 Df(2L)spd Df(2R)Jp1 Df(3L)29A6 Df(3L)Cat Df(3L)HR232 Df(3L)HR370 Df(3L)ri-79c Df(3L)XS572 Df(3R)3450 Df(3R)Antp17 Df(3R)by10 Df(3R)Cha7 Df(3R)M-Kx1 Df(3R)p40 Df(3R)p712 Df(3R)Tpl10
Alleles (107)
	14-3-3ε^EP3-732 AnxB9^EP3-562 ap^EP2-1583 ap^md544 ap^Scer\UAS.cMa ap^UGO35 Avic\GFP^{EGFP.Bx.3'UTR.αTub84B} Bx¹ caps^65.2 caps^Scer\UAS.cSa cbt^EP2-408 cbt^EP2237 cbt^EP2237E1 cbt^EP2237E28 CG1943^EP3-797 CG8369^EP3-826 CG8405^EP2-865 CG11399^EP3-28 CG11399^EY11352 chm^EP2-1069 cic^D49 cic^EP3-1005 drpr^EP2-867 drpr^EP522 drpr^Δ5 drpr^Δ19 dx^ENU Ecol\lacZ^ap-rK568 EP2-430^EP2-430 EP2-760^EP2-760 EP2-1080^EP2-1080 EP3-378^EP3-378 EP3-612^EP3-612 EP3-934^EP3-934 EP3-980^EP3-980 EP3-1199^EP3-1199 EP3-1729^EP3-1729 EP3-1789^EP3-1789 EP3-1809^EP3-1809 fng⁸⁰ fng^EP3-511 fng^EP3-581 fng^Scer\UAS.cKa H^E31 l(3)04708⁰⁴⁷⁰⁸ lilli^4U5 lilli⁰⁰⁶³² lilli^EP2-27 lilli^Scer\UAS.cWa mir-14^EP2-235 mir-14^EP2-356 mir-14^EP2-402 mir-14^EP2-814 mir-14^EP2-1142 mir-14^{Scer\UAS.T:Disc\RFP-DsRed2} mir-14^Δ1 mir-279^EP3-364 mir-282^EP3-26 Mpcp⁰⁰⁵⁶⁴ Mpcp^EP3-488 N^Ax-M1 N^nd-1 nmd^EP2-446 nmd^k10909 nmd^ry4 nuf^EP3-1715 nuf^KG02305 osa³⁰⁸ osa^EP3-473 osa^EP3-619 osa^EP3-900 osa^EP3-941 osa^EP3-1074 osa^EP3-1386 osa^EP3-1591 osa^Scer\UAS.cCa pnt^EP3-637 pnt^EP3-853 pnt^EP3-1638 pnt^EP3-1859 pnt^P2.Scer\UAS pnt^Δ88 sbb⁶ sbb^EP2-473 sbb^Scer\UAS.cTa Scer\GAL4^ap-md544 Scer\GAL4^ptc-559.1 shn¹ shn⁰⁴⁷³⁸ shn^EP2-1279 shn^EP2-1743 shn^EP2359 skd^EP3-532 skd^EP3375 skd^L7062 skd^rK760 Slh^EP2-18 Su(Tpl)¹⁷ Su(Tpl)^EP3-2015 Su(Tpl)^S-192 T:Disc\RFP^DsRed2 tara¹ tara⁰³⁸⁸¹ tara^EP3-1575 tara^EP3463 trn^28.4 trn^Scer\UAS.cMa
Constructs (11)
	P{EP} P{tub-EGFP.Bx.3'UTR} P{UAS-ap.M} P{UAS-caps.S} P{UAS-dsRed2-mir-14.B} P{UAS-fng.K} P{UAS-lilli.W} P{UAS-osa} P{UAS-pnt.P2} P{UAS-sbb.T} P{UAS-trn.M}
Genes (59)
	14-3-3ε AnxB9 ap Avic\GFP Bx caps cbt CG1943 CG4477 CG5890 CG8149 CG8369 CG8405 CG11399 CG14073 chm cic ct drpr dx E(spl)mγ-HLH Ecol\lacZ EP2-430 EP2-760 EP2-1080 EP3-378 EP3-612 EP3-934 EP3-980 EP3-1199 EP3-1729 EP3-1789 EP3-1809 fng H l(2)08717 l(3)04708 lilli mir-14 mir-279 mir-282 Mpcp Mrp4 N nmd nuf osa pnt sbb Scer\GAL4 shn skd Slh Su(Tpl) tara th trn wg γSnap
Insertions (62)
	P{EP}14-3-3ε^EP3-732 P{EP}AnxB9^EP3-562 P{EP}ap^EP2-1583 P{EP}cbt^EP2-408 P{EP}cbt^EP2237 P{EP}CG1943^EP3-797 P{EP}CG8369^EP3-826 P{EP}CG8405^EP2-865 P{EP}CG11399^EP3-28 P{EP}chm^EP2-1069 P{EP}cic^EP3-1005 P{EP}drpr^EP2-867 P{EP}drpr^EP522 P{EP}EP2-430^EP2-430 P{EP}EP2-760^EP2-760 P{EP}EP2-1080^EP2-1080 P{EP}EP3-378^EP3-378 P{EP}EP3-612^EP3-612 P{EP}EP3-934^EP3-934 P{EP}EP3-980^EP3-980 P{EP}EP3-1199^EP3-1199 P{EP}EP3-1729^EP3-1729 P{EP}EP3-1789^EP3-1789 P{EP}EP3-1809^EP3-1809 P{EP}fng^EP3-511 P{EP}fng^EP3-581 P{EP}lilli^EP2-27 P{EP}mir-14^EP2-235 P{EP}mir-14^EP2-356 P{EP}mir-14^EP2-402 P{EP}mir-14^EP2-814 P{EP}mir-14^EP2-1142 P{EP}mir-279^EP3-364 P{EP}mir-282^EP3-26 P{EP}Mpcp^EP3-488 P{EP}nmd^EP2-446 P{EP}nuf^EP3-1715 P{EP}osa^EP3-473 P{EP}osa^EP3-619 P{EP}osa^EP3-900 P{EP}osa^EP3-941 P{EP}osa^EP3-1074 P{EP}osa^EP3-1386 P{EP}osa^EP3-1591 P{EP}pnt^EP3-637 P{EP}pnt^EP3-853 P{EP}pnt^EP3-1638 P{EP}pnt^EP3-1859 P{EP}sbb^EP2-473 P{EP}shn^EP2-1279 P{EP}shn^EP2-1743 P{EP}shn^EP2359 P{EP}skd^EP3-532 P{EP}skd^EP3375 P{EP}Slh^EP2-18 P{EP}Su(Tpl)^EP3-2015 P{EP}tara^EP3-1575 P{EP}tara^EP3463 P{EPgy2}CG11399^EY11352 P{GawB}ap^md544 P{GawB}ptc^559.1 P{PZ}ap^rK568
Natural transposons (1)
	P-element