Reference Report

Reference
Citation	Cinnamon, E., Helman, A., Schyr, R.B.H., Orian, A., Jiménez, G., Paroush, Z. (2008). Multiple RTK pathways downregulate Groucho-mediated repression in Drosophila embryogenesis. Development 135(5): 829--837. (Export to RIS)
FlyBase ID	FBrf0202757
Publication Type	Research paper
PubMed ID	18216172
PubMed Abstract	RTK pathways establish cell fates in a wide range of developmental processes. However, how the pathway effector MAPK coordinately regulates the expression of multiple target genes is not fully understood. We have previously shown that the EGFR RTK pathway causes phosphorylation and downregulation of Groucho, a global co-repressor that is widely used by many developmentally important repressors for silencing their various targets. Here, we use specific antibodies that reveal the dynamics of Groucho phosphorylation by MAPK, and show that Groucho is phosphorylated in response to several RTK pathways during Drosophila embryogenesis. Focusing on the regulation of terminal patterning by the Torso RTK pathway, we demonstrate that attenuation of Groucho's repressor function via phosphorylation is essential for the transcriptional output of the pathway and for terminal cell specification. Importantly, Groucho is phosphorylated by an efficient mechanism that does not alter its subcellular localisation or decrease its stability; rather, modified Groucho endures long after MAPK activation has terminated. We propose that phosphorylation of Groucho provides a widespread, long-term mechanism by which RTK signals control target gene expression.
DOI	10.1242/dev.015206
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Development
Title	Development
Publication Year	1987-
ISBN/ISSN	0950-1991
Data from Reference
Aberrations (1)
	Df(3R)Espl22
Alleles (12)
	Dsor1^LH110 Egfr^f2 gro^{AA.Scer\UAS.P\T} gro^{DD.Scer\UAS.P\T} gro^E48 gro^{Scer\UAS.P\T.cCa} os^upd-1 Scer\GAL4^btl.PS Scer\GAL4^{nos.UTR.T:Hsim\VP16} tor^12D tsl⁴ w^+mC
Constructs (5)
	P{GAL4::VP16-nos.UTR} P{GAL4-btl.S} P{UASp-gro.AA} P{UASp-gro.C} P{UASp-gro.DD}
Genes (18)
	cic Dsor1 Egfr eve gro hb hkb kni Lam nos os rl Scer\GAL4 Stat92E tll tor tsl w
Natural transposons (1)
	P-element