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Reference
Citation
Nicholson, L., Singh, G.K., Osterwalder, T., Roman, G.W., Davis, R.L., Keshishian, H. (2008). Spatial and temporal control of gene expression in Drosophila using the inducible GeneSwitch GAL4 System. I. Screen for larval nervous system drivers.  Genetics 178(1): 215--234.
FlyBase ID
FBrf0202785
Publication Type
Research paper
Abstract

There is a critical need for genetic methods for the inducible expression of transgenes in specific cells during development. A promising approach for this is the GeneSwitch GAL4 system of Drosophila. With GeneSwitch GAL4 the expression of upstream activating sequence (UAS) effector lines is controlled by a chimeric GAL4 protein that becomes active in the presence of the steroid RU486 (mifepristone). To improve the utility of this expression system, we performed a large-scale enhancer-trap screen for insertions that yielded nervous system expression. A total of 204 GeneSwitch GAL4 lines with various larval expression patterns in neurons, glia, and/or muscle fibers were identified for chromosomes I-III. All of the retained lines show increased activity when induced with RU486. Many of the lines reveal novel patterns of sensory neurons, interneurons, and glia. There were some tissue-specific differences in background expression, with muscles and glia being more likely to show activity in the absence of the inducing agent. However, >90% of the neuron-specific driver lines showed little or no background activity, making them particularly useful for inducible expression studies.

PubMed ID
PubMed Central ID
PMC2206072 (PMC) (EuropePMC)
Related Publication(s)
Personal communication to FlyBase

Additional mapping and expression information for GeneSwitch lines from FBrf0202785.
Cole et al., 2012.7.5, Additional mapping and expression information for GeneSwitch lines from FBrf0202785. [FBrf0218802]

Molecular mapping information for GeneSwitch lines from FBrf0202785.
Cole et al., 2012.7.6, Molecular mapping information for GeneSwitch lines from FBrf0202785. [FBrf0219065]

Associated Information
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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Genetics
    Title
    Genetics
    Publication Year
    1916-
    ISBN/ISSN
    0016-6731
    Data From Reference
    Alleles (68)
    Genes (54)
    Insertions (51)
    Transgenic Constructs (2)