Reference Report

Reference
Citation	Nicholson, L., Singh, G.K., Osterwalder, T., Roman, G.W., Davis, R.L., Keshishian, H. (2008). Spatial and temporal control of gene expression in Drosophila using the inducible GeneSwitch GAL4 System. I. Screen for larval nervous system drivers. Genetics 178(1): 215--234. (Export to RIS)
FlyBase ID	FBrf0202785
Publication Type	Research paper
PubMed ID	18202369
PubMed Abstract	There is a critical need for genetic methods for the inducible expression of transgenes in specific cells during development. A promising approach for this is the GeneSwitch GAL4 system of Drosophila. With GeneSwitch GAL4 the expression of upstream activating sequence (UAS) effector lines is controlled by a chimeric GAL4 protein that becomes active in the presence of the steroid RU486 (mifepristone). To improve the utility of this expression system, we performed a large-scale enhancer-trap screen for insertions that yielded nervous system expression. A total of 204 GeneSwitch GAL4 lines with various larval expression patterns in neurons, glia, and/or muscle fibers were identified for chromosomes I-III. All of the retained lines show increased activity when induced with RU486. Many of the lines reveal novel patterns of sensory neurons, interneurons, and glia. There were some tissue-specific differences in background expression, with muscles and glia being more likely to show activity in the absence of the inducing agent. However, >90% of the neuron-specific driver lines showed little or no background activity, making them particularly useful for inducible expression studies.
DOI	10.1534/genetics.107.081968
Related Publication(s)
Supplementary material	Supplemental Table 3. GeneSwitch GAL4 line expression patterns. [FBrf0218769]
Personal communication to FlyBase	Additional mapping and expression information for GeneSwitch lines from FBrf0202785. Cole et al., 2012.7.5, Additional mapping and expression information for GeneSwitch lines from FBrf0202785. [FBrf0218802] Molecular mapping information for GeneSwitch lines from FBrf0202785. Cole et al., 2012.7.6, Molecular mapping information for GeneSwitch lines from FBrf0202785. [FBrf0219065]
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Genetics
Title	Genetics
Publication Year	1916-
ISBN/ISSN	0016-6731
Data from Reference
Alleles (68)
	Atpα^GSG3285-1 Avic\GFP^{2x.EGFP.Scer\UAS} CG2765^GSG8708 CG10082^GSG8942 CG15312^GSG3330-2 CG30015^GSG4356 CG31619^GSG1802-2 cpo^GSGB38b3-2 cpo^GSGB62b2-1 kuz^GSG4064-1 l(2)GSG317^GSG317 Lk6^GSG1142 pum^GSGB13-7 Roc2^GSG10703 Scer\GAL4^{amn-GSG3173-3} Scer\GAL4^{Atpα-GSG3285-1} Scer\GAL4^{bon-GSG2451-1} Scer\GAL4^bun-GSG5961 Scer\GAL4^{CG2765-GSG8708} Scer\GAL4^{CG10082-GSG8942} Scer\GAL4^{CG15312-GSG3330-2} Scer\GAL4^{CG30015-GSG4356} Scer\GAL4^{CG31619-GSG1802-2} Scer\GAL4^cpo-GSG125 Scer\GAL4^{cpo-GSGB38b3-2} Scer\GAL4^{cpo-GSGB62b2-1} Scer\GAL4^GSG12-1 Scer\GAL4^GSG176 Scer\GAL4^GSG329-1 Scer\GAL4^GSG734 Scer\GAL4^GSG907 Scer\GAL4^GSG1201-2 Scer\GAL4^GSG1202-2 Scer\GAL4^GSG1687 Scer\GAL4^GSG2295 Scer\GAL4^GSG2553 Scer\GAL4^GSG2935 Scer\GAL4^GSG3147-1 Scer\GAL4^GSG3183 Scer\GAL4^GSG3315-1 Scer\GAL4^GSG3473 Scer\GAL4^GSG3516 Scer\GAL4^GSG3621-1 Scer\GAL4^GSG3630 Scer\GAL4^GSG3678 Scer\GAL4^GSG3705 Scer\GAL4^GSG4102 Scer\GAL4^GSG5722 Scer\GAL4^GSG6098 Scer\GAL4^GSG6124 Scer\GAL4^GSG8302-2 Scer\GAL4^GSG8892 Scer\GAL4^GSGB12-6 Scer\GAL4^GSGB15-1 Scer\GAL4^{HDAC4-GSG3938} Scer\GAL4^{kuz-GSG4064-1} Scer\GAL4^{l(2)GSG317-GSG317} Scer\GAL4^Lk6-GSG1142 Scer\GAL4^{MTF-1-GSGB3b1-3} Scer\GAL4^pum-GSGB13-7 Scer\GAL4^{Roc2-GSG10703} Scer\GAL4^{sky-GSGB94b1-1} Scer\GAL4^tou-GSG2797 Scer\GAL4^{Tsp42Ef-GSG5255} Scer\GAL4^{βTub56D-GSG5793} sky^GSGB94b1-1 Tsp42Ef^GSG5255 βTub56D^GSG5793
Constructs (2)
	P{Switch2} P{UAS-2xEGFP}
Genes (54)
	amn Atpα Avic\GFP Cad86C Ccs CG2765 CG4250 CG4565 CG5790 CG6567 CG7794 CG8003 CG9005 CG10082 CG15312 CG30015 CG31619 CG32066 CG32710 CG42565 CG42566 cpo E(bx) egr Fas3 gem Gp210 hoip Hsrω htl kibra kuz l(2)GSG317 Lim1 Lk6 Pka-C1 pum RapGAP1 repo RhoL RluA-1 Roc2 Scer\GAL4 sky snRNA:U1:95Cb snRNA:U1:95Cc Spn28B ss stas Tret1-1 tRNA:G3:35Ba Tsp42Ef X11L βTub56D
Insertions (51)
	P{Switch2}amn^GSG3173-3 P{Switch2}Atpα^GSG3285-1 P{Switch2}bon^GSG2451-1 P{Switch2}bun^GSG5961 P{Switch2}CG2765^GSG8708 P{Switch2}CG10082^GSG8942 P{Switch2}CG15312^GSG3330-2 P{Switch2}CG30015^GSG4356 P{Switch2}CG31619^GSG1802-2 P{Switch2}cpo^GSG125 P{Switch2}cpo^GSGB38b3-2 P{Switch2}cpo^GSGB62b2-1 P{Switch2}GSG12-1 P{Switch2}GSG176 P{Switch2}GSG329-1 P{Switch2}GSG734 P{Switch2}GSG907 P{Switch2}GSG1201-2 P{Switch2}GSG1202-2 P{Switch2}GSG1687 P{Switch2}GSG2295 P{Switch2}GSG2553 P{Switch2}GSG2935 P{Switch2}GSG3147-1 P{Switch2}GSG3183 P{Switch2}GSG3315-1 P{Switch2}GSG3473 P{Switch2}GSG3516 P{Switch2}GSG3621-1 P{Switch2}GSG3630 P{Switch2}GSG3678 P{Switch2}GSG3705 P{Switch2}GSG4102 P{Switch2}GSG5722 P{Switch2}GSG6098 P{Switch2}GSG6124 P{Switch2}GSG8302-2 P{Switch2}GSG8892 P{Switch2}GSGB12-6 P{Switch2}GSGB15-1 P{Switch2}HDAC4^GSG3938 P{Switch2}kuz^GSG4064-1 P{Switch2}l(2)GSG317^GSG317 P{Switch2}Lk6^GSG1142 P{Switch2}MTF-1^GSGB3b1-3 P{Switch2}pum^GSGB13-7 P{Switch2}Roc2^GSG10703 P{Switch2}sky^GSGB94b1-1 P{Switch2}tou^GSG2797 P{Switch2}Tsp42Ef^GSG5255 P{Switch2}βTub56D^GSG5793