Reference Report

Reference
Citation	Wu, C.L., Xia, S., Fu, T.F., Wang, H., Chen, Y.H., Leong, D., Chiang, A.S., Tully, T. (2007). Specific requirement of NMDA receptors for long-term memory consolidation in Drosophila ellipsoid body. Nat. Neurosci. 10(12): 1578--1586. (Export to RIS)
FlyBase ID	FBrf0202786
Publication Type	Research paper
PubMed ID	17982450
PubMed Abstract	In humans and many other animals, memory consolidation occurs through multiple temporal phases and usually involves more than one neuroanatomical brain system. Genetic dissection of Pavlovian olfactory learning in Drosophila melanogaster has revealed multiple memory phases, but the predominant view holds that all memory phases occur in mushroom body neurons. Here, we demonstrate an acute requirement for NMDA receptors (NMDARs) outside of the mushroom body during long-term memory (LTM) consolidation. Targeted dsRNA-mediated silencing of Nmdar1 and Nmdar2 (also known as dNR1 or dNR2, respectively) in cholinergic R4m-subtype large-field neurons of the ellipsoid body specifically disrupted LTM consolidation, but not retrieval. Similar silencing of functional NMDARs in the mushroom body disrupted an earlier memory phase, leaving LTM intact. Our results clearly establish an anatomical site outside of the mushroom body involved with LTM consolidation, thus revealing both a distributed brain system subserving olfactory memory formation and the existence of a system-level memory consolidation in Drosophila.
DOI	10.1038/nn2005
Related Publication(s)
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Associated Information
Comments
Associated Files
Other Information
Secondary IDs
Language of Publication	English
Additional Languages of Abstract
Also Published As
Parent Publication
Publication Type	Journal
Abbreviation	Nat. Neurosci.
Title	Nature Neuroscience
Publication Year	1998-
ISBN/ISSN	1097-6256
Data from Reference
Alleles (10)
	Mmus\Cd8a^{Scer\UAS.T:Avic\GFP} Nmdar1^{dsRNA.Scer\UAS.cWa} Nmdar2^{dsRNA.Scer\UAS.cWa} Nmdar2^Scer\UAS.cWa Scer\GAL4^elav-C155 Scer\GAL4^ey-OK107 Scer\GAL4^Feb170 Scer\GAL4^hs.PU Scer\GAL80^ts.αTub84B shi^1.Scer\UAS
Constructs (7)
	P{hs-GAL4.U} P{tubP-GAL80^ts} P{UAS-mCD8::GFP.L} P{UAS-Nmdar1.dsRNA} P{UAS-Nmdar2.dsRNA} P{UAS-Nmdar2.W} P{UAS-shi^ts1.K}
Genes (7)
	CG17124 Mmus\Cd8a Nmdar1 Nmdar2 Scer\GAL4 Scer\GAL80 shi
Insertions (3)
	P{GawB}elav^C155 P{GawB}ey^OK107 P{GawB}Feb170
Natural transposons (1)
	P-element
Transcripts (1)
	Scer\GAL4^Feb170RA