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Schuldiner, O., Berdnik, D., Levy, J.M., Wu, J.S., Luginbuhl, D., Gontang, A.C., Luo, L. (2008). piggyBac-based mosaic screen identifies a postmitotic function for cohesin in regulating developmental axon pruning.  Dev. Cell 14(2): 227--238.
FlyBase ID
FBrf0202859
Publication Type
Research paper
Abstract

Developmental axon pruning is widely used to refine neural circuits. We performed a mosaic screen to identify mutations affecting axon pruning of Drosophila mushroom body gamma neurons. We constructed a modified piggyBac vector with improved mutagenicity and generated insertions in >2000 genes. We identified two cohesin subunits (SMC1 and SA) as being essential for axon pruning. The cohesin complex maintains sister-chromatid cohesion during cell division in eukaryotes. However, we show that the pruning phenotype in SMC1(-/-) clones is rescued by expressing SMC1 in neurons, revealing a postmitotic function. SMC1(-/-) clones exhibit reduced levels of the ecdysone receptor EcR-B1, a key regulator of axon pruning. The pruning phenotype is significantly suppressed by overexpressing EcR-B1 and is enhanced by a reduced dose of EcR, supporting a causal relationship. We also demonstrate a postmitotic role for SMC1 in dendrite targeting of olfactory projection neurons. We suggest that cohesin regulates diverse aspects of neuronal morphogenesis.

PubMed ID
PubMed Central ID
PMC2268086 (PMC) (EuropePMC)
Related Publication(s)
Supplementary material
Personal communication to FlyBase

Insertions of PBac{SAstopDsRed} GSS accessions.
Schuldiner et al., 2009, Insertions of PBac{SAstopDsRed} GSS accessions. [FBrf0208716]

Note

Running rings around chromosomes to trim axons and target dendrites.
Dorsett, 2008, Dev. Cell 14(2): 156--158 [FBrf0216019]

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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Dev. Cell
    Title
    Developmental Cell
    Publication Year
    2001-
    ISBN/ISSN
    1534-5807 1878-1551
    Data From Reference