Reference Report

Reference
Citation	Mortimer, N.T., Moberg, K.H. (2007). The Drosophila F-box protein Archipelago controls levels of the Trachealess transcription factor in the embryonic tracheal system. Dev. Biol. 312(2): 560--571. (Export to RIS)
FlyBase ID	FBrf0202878
Publication Type	Research paper
PubMed ID	17976568
PubMed Abstract	The archipelago gene (ago) encodes the F-box specificity subunit of an SCF(skp-cullin-f box) ubiquitin ligase that inhibits cell proliferation in Drosophila melanogaster and suppresses tumorigenesis in mammals. ago limits mitotic activity by targeting cell cycle and cell growth proteins for ubiquitin-dependent degradation, but the diverse developmental roles of other F-box proteins suggests that it is likely to have additional protein targets. Here we show that ago is required for the post-mitotic shaping of the Drosophila embryonic tracheal system, and that it acts in this tissue by targeting the Trachealess (Trh) protein, a conserved bHLH-PAS transcription factor. ago restricts Trh levels in vivo and antagonizes transcription of the breathless FGF receptor, a known target of Trh in the tracheal system. At a molecular level, the Ago protein binds Trh and is required for proteasome-dependent elimination of Trh in response to expression of the Dysfusion protein. ago mutations that elevate Trh levels in vivo are defective in binding forms of Trh found in Dysfusion-positive cells. These data identify a novel function for the ago ubiquitin-ligase in tracheal morphogenesis via Trh and its target breathless, and suggest that ago has distinct functions in mitotic and post-mitotic cells that influence its role in development and disease.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Dev. Biol.
Title	Developmental Biology
Publication Year	1959-
ISBN/ISSN	0012-1606
Data from Reference
Aberrations (1)
	Df(3L)Exel9000
Alleles (17)
	ago¹ ago³ ago^Scer\UAS.cMa ago^{ΔF.MtnA.T:Ivir\HA1} ago^ΔF.Scer\UAS awd^j2A4 btl^dev1 btl^EY01638 CycE^Scer\UAS.cUa dm^Scer\UAS.cZa Ecol\lacZ^esg-G66 Ecol\lacZ^trh-1-eve-1 Prosβ2^1.Scer\UAS Prosβ6^1.B.Scer\UAS Scer\GAL4^btl.PS trh¹⁰⁵¹² trh^{Scer\UAS.T:Zzzz\FLAG}
Constructs (8)
	P{GAL4-btl.S} P{UAS-ago.M} P{UAS-ago.ΔF} P{UAS-CycE.U} P{UAS-dm.Z} P{UAS-Pros26¹.B} P{UAS-Prosβ2¹} P{UAS-trh.FLAG}
Genes (14)
	ago awd btl CycE dm dys Dys Ecol\lacZ esg Prosβ2 Prosβ6 Scer\GAL4 tgo trh
Insertions (5)
	P{enG}esg^G66 P{EPgy2}btl^EY01638 P{ET-L}trh-1-eve-1 P{lacW}awd^j2A4 P{PZ}trh¹⁰⁵¹²
Natural transposons (1)
	P-element