Reference Report

Reference
Citation	Song, J., Tanouye, M. (2007). Role for para sodium channel gene 3' UTR in the modification of Drosophila seizure susceptibility. Dev. Neurobiol. 67(14): 1944--1956. (Export to RIS)
FlyBase ID	FBrf0202966
Publication Type	Research paper
PubMed ID	17918245
PubMed Abstract	Voltage-gated sodium channel genes are highly regulated at the level of transcription or translation. In this study, we have utilized the combination of genetic, electrophysiological, and molecular analyses to identify a 7-kb 3'-untranslated region (UTR) of the Drosophila para sodium channel gene. Disruption of this segment by P-element insertion causes reduction of para primary transcript, but not Rbp2 transcripts. The identification of this novel 3'-UTR is based on a P-insertion mutation called para(JS1), which was isolated from a P-element mutagenesis screen for seizure suppressors in a Drosophila model of epilepsy. The para(JS1) mutation was identified 6845 bp downstream of the para gene, which resides in an intergenic region that lies between para and Rbp2 (RNA-binding protein 2) genes. Interestingly, reverse-transcription PCR showed that the region containing para(JS1) is substantially transcribed and this transcribed region is associated with para coding region. We discussed possible mechanisms of how reduced transcription of the para gene or alterations in sodium channel subunit composition might be indicated by the para(JS1) mutation and implications for para 3' UTR function.
DOI	10.1002/dneu.20519
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Dev. Neurobiol.
Title	Developmental Neurobiology
Publication Year	2007--
ISBN/ISSN	1932-8451 1932-846X
Data from Reference
Aberrations (2)
	Df(1)D34 Tp(1;2)r⁺75c
Alleles (32)
	eas² mle^nap-ts1 para^{13.5.Scer\UAS} para^hd5 para^ID7 para^ID30 para^JS1 para^JS1-Ex1 para^JS1-Ex2 para^JS1-Ex3 para^JS1-Ex4 para^JS1-Ex5 para^JS1-Ex6 para^JS1-Ex7 para^JS1-Ex8 para^JS1-Ex9 para^JS1-Ex10 para^JS1-Ex11 para^JS1-Ex12 para^JS1-Ex13 para^JS1-Ex14 para^JS1-Ex15 para^JS1-Ex16 para^JS1-Ex18 para^lk2 para^Scer\UAS.cGa para^ST76 para^ts1 para^ts115 Rbp2^EY00852 Scer\GAL4^elav.PLu sda^iso7.8
Constructs (5)
	P{?lacW} P{GAL4-elav.L} P{lacW} P{UAS-para.13.5} P{UAS-para.cGa}
Genes (6)
	eas mle para Rbp2 Scer\GAL4 sda
Insertions (17)
	P{?lacW}para^JS1-Ex1 P{?lacW}para^JS1-Ex2 P{?lacW}para^JS1-Ex3 P{?lacW}para^JS1-Ex4 P{?lacW}para^JS1-Ex5 P{?lacW}para^JS1-Ex6 P{?lacW}para^JS1-Ex7 P{?lacW}para^JS1-Ex8 P{?lacW}para^JS1-Ex9 P{?lacW}para^JS1-Ex10 P{?lacW}para^JS1-Ex11 P{?lacW}para^JS1-Ex12 P{?lacW}para^JS1-Ex13 P{?lacW}para^JS1-Ex14 P{?lacW}para^JS1-Ex15 P{?lacW}para^JS1-Ex16 P{lacW}para^JS1
Natural transposons (1)
	P-element