Reference Report

Reference
Citation	Okamura, K., Phillips, M.D., Tyler, D.M., Duan, H., Chou, Y.T., Lai, E.C. (2008). The regulatory activity of microRNA* species has substantial influence on microRNA and 3' UTR evolution. Nat. Struct. Mol. Biol. 15(4): 354--363. (Export to RIS)
FlyBase ID	FBrf0204314
Publication Type	Research paper
PubMed ID	18376413
PubMed Abstract	During microRNA (miRNA) biogenesis, one strand of a approximately 21-22-nucleotide RNA duplex is preferentially selected for entry into a silencing complex. The other strand, known as the miRNA* species, has typically been assumed to be a carrier strand. Here we show that, although Drosophila melanogaster miRNA* species are less abundant than their partners, they are often present at physiologically relevant levels and can associate with Argonaute proteins. Comparative genomic analyses revealed that >40% of miRNA* sequences resist nucleotide divergence across Drosophilid evolution, and at least half of these well-conserved miRNA* species select for conserved 3' untranslated region seed matches well above background noise. Finally, we validated the inhibitory activity of miRNA* species in both cultured cells and transgenic animals. These data broaden the reach of the miRNA regulatory network and suggest an important mechanism that diversifies miRNA function during evolution.
DOI	10.1038/nsmb.1409
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Secondary IDs
Language of Publication	English
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Parent Publication
Publication Type	Journal
Abbreviation	Nat. Struct. Mol. Biol.
Title	Nature Structural and Molecular Biology
Publication Year	2004-
ISBN/ISSN	1545-9993 1545-9985
Data from Reference
Alleles (8)
	Avic\GFP^{EGFP.ab.3'UTR.αTub84B} Avic\GFP^{miR-iab4-3p.tub} Avic\GFP^{miR-iab4-5p.tub} Avic\GFP^{miR-iab8-3p.tub} Avic\GFP^{miR-iab8-5p.tub} mir-iab-4^{Scer\UAS.cRa.T:Disc\RFP-DsRed2} mir-iab-8^{Scer\UAS.cTa.T:Disc\RFP-DsRed2} Scer\GAL4^ptc-559.1
Constructs (7)
	P{tub-EGFP.ab.3'UTR} P{tub-GFP.mir-iab4-3p} P{tub-GFP.mir-iab4-5p} P{tub-GFP.mir-iab8-3p} P{tub-GFP.mir-iab8-5p} P{UAS-DsRed2-mir-iab-4.R} P{UAS-DsRed-mir-iab-8.T}
Genes (32)
	AGO1 Avic\GFP ban mir-2a-2 mir-2b-2 mir-3 mir-4 mir-5 mir-6-1 mir-6-2 mir-6-3 mir-7 mir-8 mir-10 mir-34 mir-92a mir-184 mir-276a mir-276b mir-281-1 mir-282 mir-286 mir-288 mir-306 mir-309 mir-315 mir-996 mir-1003 mir-1010 mir-iab-4 mir-iab-8 Scer\GAL4
Insertions (1)
	P{GawB}ptc^559.1
Natural transposons (1)
	P-element