Reference Report

Reference
Citation	Johnson, R.I., Seppa, M.J., Cagan, R.L. (2008). The Drosophila CD2AP/CIN85 orthologue Cindr regulates junctions and cytoskeleton dynamics during tissue patterning. J. Cell Biol. 180(6): 1191--1204. (Export to RIS)
FlyBase ID	FBrf0204348
Publication Type	Research paper
PubMed ID	18362180
PubMed Abstract	Developing tissues require cells to undergo intricate processes to shift into appropriate niches. This requires a functional connection between adhesion-mediating events at the cell surface and a cytoskeletal reorganization to permit directed movement. A small number of proteins are proposed to link these processes. Here, we identify one candidate, Cindr, the sole Drosophila melanogaster member of the CD2AP/CIN85 family (this family has been previously implicated in a variety of processes). Using D. melanogaster retina, we demonstrate that Cindr links cell surface junctions (E-cadherin) and adhesion (Roughest) with multiple components of the actin cytoskeleton. Reducing cindr activity leads to defects in local cell movement and, consequently, tissue patterning and cell death. Cindr activity is required for normal localization of Drosophila E-cadherin and Roughest, and we show additional physical and functional links to multiple components of the actin cytoskeleton, including the actin-capping proteins capping protein alpha and capping protein beta. Together, these data demonstrate that Cindr is involved in dynamic cell rearrangement in an emerging epithelium.
DOI	10.1083/jcb.200706108
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Language of Publication	English
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Publication Type	Journal
Abbreviation	J. Cell Biol.
Title	Journal of Cell Biology
Publication Year	1966-
ISBN/ISSN	0021-9525
Data from Reference
Aberrations (2)
	Df(3R)3450 In(1)rst³
Alleles (45)
	Abl² Arp3^EP3640 Avic\GFP^{S65T.Scer\UAS} Avic\GFP^Scer\UAS.cUa chic²²¹ cindr^{dsRNA.PC.PD.Scer\UAS} cindr^{dsRNA.PC.Scer\UAS} cindr^{dsRNA.Scer\UAS} cort^D4 cort^M7 cpa^69E cpa^107E cpa^{Scer\UAS.T:Ivir\HA1} cpa^scrd1 cpa^scrd2 cpb^6.15 cpb^bnd1 cpb^F19 cpb^M143 cpb^Scer\UAS.cWa Ecol\lacZ^Scer\UAS.cUa hbs³⁶¹ hbs⁴⁵⁹ hbs^{dsRNA.Scer\UAS} Mtl^Δ Rac1^J11 Rac2^Δ Rho1^72R rst⁶ rst^{dsRNA.Scer\UAS} rst^Scer\UAS.cBa SCAR^k03107 SCAR^Δ37 Scer\GAL4^ap-md544 Scer\GAL4^da.G32 Scer\GAL4^GMR.PF Scer\GAL4^ptc-559.1 Scer\GAL4^{Scer\FRT.Act5C} Scer\GAL4^tub.PU shg^R69 shg^{Scer\UAS.T:Avic\GFP-rs} tsr^N96A tsr^N121 WASp³ α-Cat^{Scer\UAS.T:Avic\GFP-sg}
Constructs (16)
	P{AyGAL4} P{GAL4-da.G32} P{GAL4-ninaE.GMR} P{tub-GAL4.U} P{UAS-cindr.IR} P{UAS-cindr-PC.IR} P{UAS-cindr-PC-PD.IR} P{UAS-cpa.HA} P{UAS-cpb.W} P{UAS-GFP.S65T} P{UAS-GFP.U} P{UAS-hbs.dsRNA} P{UAS-lacZ.U} P{UAS-rst.dsRNA} P{UAS-shg.DEFL} P{UAS-α-Cat.T:GFP.sg}
Genes (21)
	Abl Arp3 Avic\GFP chic cindr cort cpa cpb Ecol\lacZ hbs Mtl Rac1 Rac2 Rho1 rst SCAR Scer\GAL4 shg tsr WASp α-Cat
Insertions (3)
	P{EP}Arp3^EP3640 P{GawB}ap^md544 P{GawB}ptc^559.1