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Citation
Murawska, M., Kunert, N., van Vugt, J., L√§ngst, G., Kremmer, E., Logie, C., Brehm, A. (2008). dCHD3, a novel ATP-dependent chromatin remodeler associated with sites of active transcription.  Mol. Cell. Biol. 28(8): 2745--2757.
FlyBase ID
FBrf0204378
Publication Type
Research paper
Abstract

ATP-dependent chromatin remodelers of the CHD family play important roles during differentiation and development. Three CHD proteins, dMi-2, dChd1, and Kismet, have been described for Drosophila melanogaster. Here, we study dCHD3, a novel member of the CHD family. dCHD3 is related in sequence to dMi-2 but lacks several domains implicated in dMi-2 function. We demonstrate that dCHD3 is a nuclear protein and that expression is tightly regulated during fly development. Recombinant dCHD3 remodels mono- and polynucleosomes in an ATP-dependent manner in vitro. Its chromodomains are critical for nucleosome binding and remodeling. Unlike dMi-2, dCHD3 exists as a monomer. Nevertheless, both proteins colocalize with RNA polymerase II to actively transcribed regions on polytene chromosomes, suggesting that both remodelers participate in the process of transcription.

PubMed ID
PubMed Central ID
PMC2293103 (PMC) (EuropePMC)
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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Mol. Cell. Biol.
    Title
    Molecular and Cellular Biology
    Publication Year
    1981-
    ISBN/ISSN
    0270-7306
    Data From Reference
    Genes (10)
    Physical Interactions (1)
    Cell Lines (1)