Reference Report

Reference
Citation	Homem, C.C.F., Peifer, M. (2008). Diaphanous regulates myosin and adherens junctions to control cell contractility and protrusive behavior during morphogenesis. Development 135(6): 1005--1018. (Export to RIS)
FlyBase ID	FBrf0204461
Publication Type	Research paper
PubMed ID	18256194
PubMed Abstract	Formins are key regulators of actin nucleation and elongation. Diaphanous-related formins, the best-known subclass, are activated by Rho and play essential roles in cytokinesis. In cultured cells, Diaphanous-related formins also regulate cell adhesion, polarity and microtubules, suggesting that they may be key regulators of cell shape change and migration during development. However, their essential roles in cytokinesis hamper our ability to test this hypothesis. We used loss- and gain-of-function approaches to examine the role of Diaphanous in Drosophila morphogenesis. We found that Diaphanous has a dynamic expression pattern consistent with a role in regulating cell shape change. We used constitutively active Diaphanous to examine its roles in morphogenesis and its mechanisms of action. This revealed an unexpected role in regulating myosin levels and activity at adherens junctions during cell shape change, suggesting that Diaphanous helps coordinate adhesion and contractility of the underlying actomyosin ring. We tested this hypothesis by reducing Diaphanous function, revealing striking roles in stabilizing adherens junctions and inhibiting cell protrusiveness. These effects also are mediated through coordinated effects on myosin activity and adhesion, suggesting a common mechanism for Diaphanous action during morphogenesis.
DOI	10.1242/dev.016337
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Development
Title	Development
Publication Year	1987-
ISBN/ISSN	0950-1991
Data from Reference
Alleles (18)
	Act5C^{Scer\UAS.T:Avic\GFP} dia² dia⁵ dia^{CA.Scer\UAS.T:Ivir\HA1} dia^{Scer\UAS.T:Avic\GFP-EGFP} dia^unspecified Ggal\MLCK^ct.Scer\UAS Hsap\SRF^{Scer\UAS.T:Hsim\VP16} Moe^{GMA.Scer\UAS.T:Avic\GFP-S65T} Rho1^72O RhoGEF2⁰⁴²⁹¹ Scer\GAL4^c381 Scer\GAL4^e22c Scer\GAL4^en-e16E Scer\GAL4^prd.RG1 sqh^{RLC.T:Avic\GFP-S65T} T:Avic\GFP^EGFP zip¹
Constructs (8)
	P{GAL4-prd.F} P{sqh-GFP.RLC} P{SRF-VP16} P{UAS-Act5C.T:GFP} P{UAS-dia.CA} P{UAS-GMA} P{UAS-MLCK.ct} P{UASp-dia.EGFP}
Genes (19)
	Act5C arm dia dlg1 ena Ggal\MLCK Hsap\SRF Mhc Moe mys odd Rab5 Rho1 RhoGEF2 Scer\GAL4 shg sqh zip α-Cat
Insertions (3)
	P{en2.4-GAL4}e16E P{en2.4-GAL4}e22c P{GawB}c381
Natural transposons (1)
	P-element