Isolation and characterization of Df(3R)BSC460
Stacey Christensen, Jill Gresens, Kim Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(3R)BSC460 was isolated as a FLP recombinase-induced recombination event involving P{XP}Mes-4d03569 and PBac{WH}beat-VIf05694. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of w1118; Dr1/TM6C, Sb1 females crossed to P{hsFLP}1, y1 w1118; P{XP}Mes-4d03569/PBac{WH}beat-VIf05694 males. The males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC460 from the segment of P{XP}Mes-4d03569 to the left of its FRT site and the segment of PBac{WH}beat-VIf05694 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. The cytological breakpoints of Df(3R)BSC460 predicted from the Release 5 genomic coordinates of the transposable element insertions sites are 98B6;98D2. Df(3R)BSC460 failed to complement Df(3R)BSC322.