Df(3L)BSC410 was isolated as a FLP recombinase-induced recombination event involving P{XP}Usp36[d06513] and PBac{WH}CG8270[f03412]. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of w[1118]; Dr[1]/TM6C, Sb[1] females crossed to P{hsFLP}1, y[1] w[1118]; P{XP}Usp36[d06513]/PBac{WH}CG8270[f03412] males. The males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC410 from the segment of P{XP}Usp36[d06513] to the left of its FRT site and the segment of PBac{WH}CG8270[f03412] to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. The cytological breakpoints of Df(3L)BSC410 predicted from the Release 5 genomic coordinates of the transposable element insertions sites are 64E7;65B3. Df(3L)BSC410 failed to complement vn[C221] and loj[04026].