Isolation and characterization of Df(3R)BSC479
Stacey Christensen, Kim Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(3R)BSC479 was isolated as a FLP recombinase-induced recombination event involving PBac{WH}CG11870f03049 and P{XP}CG6567d09306. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of w1118; Dr1/TM6C, Sb1 females crossed to P{hsFLP}1, y1 w1118; PBac{WH}CG11870f03049/P{XP}CG6567d09306 males. The males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC479 from the segment of PBac{WH}CG11870f03049 to the left of its FRT site and the segment of P{XP}CG6567d09306 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. The cytological breakpoints of Df(3R)BSC479 predicted from the Release 5 genomic coordinates of the transposable element insertions sites are 86A3;86C7. Df(3R)BSC479 failed to complement jumu06439 and hth5E04.