Isolation and characterization of Df(3R)BSC486
Stacey Christensen, Kim Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(3R)BSC486 was isolated as a FLP recombinase-induced recombination event involving PBac{WH}CG5245f05954 and P{XP}d04743. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of w1118; Dr1/TM6C, Sb1 females crossed to P{hsFLP}1, y1 w1118; PBac{WH}CG5245f05954/P{XP}d04743 males. The males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC486 from the segment of PBac{WH}CG5245f05954 to the left of its FRT site and the segment of P{XP}d04743 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. The cytological breakpoints of Df(3R)BSC486 predicted from the Release 5 genomic coordinates of the transposable element insertions sites are 87B10;87E9. Df(3R)BSC486 failed to complement Vha5516.