Isolation and characterization of Df(3R)BSC496
Stacey Christensen, Kim Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(3R)BSC496 was isolated as a FLP recombinase-induced recombination event involving P{XP}Aldd00219 and PBac{WH}CG5500f01962. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of w1118; Dr1/TM6C, Sb1 females crossed to P{hsFLP}1, y1 w1118; P{XP}Aldd00219/PBac{WH}CG5500f01962 males. The males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC496 from the segment of P{XP}Aldd00219 to the left of its FRT site and the segment of PBac{WH}CG5500f01962 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. Exelixis, Inc. determined the insertion site of PBac{WH}CG5500f01962 to be Release 3 genomic coordinate 22691747 on chromosome arm 3R, which corresponds to 97D6 on the Release 3 genome map and 97D4 on the Release 5 genome map. The cytological breakpoints of Df(3R)BSC496 predicted from the Release 5 genomic coordinates of the transposable element insertions sites are 97A6;97D4. Df(3R)BSC496 failed to complement His2Av810 and Rb97D1.