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Christensen, S., Cook, K., Cook, K. (2008.4.15). Isolation and characterization of Df(3R)BSC500. 
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FBrf0204528
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From: 	Kevin Cook <kcook@XXXX>
To: 	flybase-updates@XXXX
Subject: 	Isolation and characterization of Df(3R)BSC500
Date: 	Tue, 15 Apr 2008  15:15:14  -0400  ( 20:15  BST)
Isolation and characterization of Df(3R)BSC500
Stacey Christensen, Kim Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(3R)BSC500 was isolated as a FLP recombinase-induced recombination 
event involving P{XP}d00762 and PBac{WH}CAP-D2[f03381]. The deletion 
was isolated as a chromosome lacking miniwhite markers in progeny of 
w[1118]; Dr[1]/TM6C, Sb[1] females crossed to P{hsFLP}1, y[1] 
w[1118]; P{XP}d00762/PBac{WH}CAP-D2[f03381] males. The males were 
heat shocked as larvae as described in Parks et al., Nature Genetics 
36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding 
and succeeding generations maintained the original genetic background 
of the Exelixis insertion stocks (Thibault et al., Nature Genetics 
36: 283-287, 2004; FBrf0175002). The recombination event generated 
the genetic element P+PBac{XP5.WH5}BSC500 from the segment of 
P{XP}d00762 to the left of its FRT site and the segment of 
PBac{WH}CAP-D2[f03381] to the right of its FRT site. Its presence was 
verified using the PCR methods and primers described in Parks et al. 
Exelixis, Inc. determined the insertion site of P{XP}d00762 to be 
Release 3 genomic coordinate 24927615 on chromosome arm 3R. This 
corresponds to 98F10 on the Release 3 and Release 5 genome maps. The 
predicted position of PBac{WH}CAP-D2[f03381] on the Release 5 map is 
99B7. Consequently, the cytological breakpoints of Df(3R)BSC500 are 
predicted to be 98F10;99B7. Df(3R)BSC500 failed to complement stg[4].
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    English
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    Aberrations (1)
    Alleles (1)
    Genes (1)
    Insertions (3)
    Transgenic Constructs (1)