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Christensen, S., Cook, K., Cook, K. (2008.4.15). Isolation and characterization of Df(3R)BSC505. 
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FBrf0204533
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From: 	Kevin Cook <kcook@XXXX>
To: 	flybase-updates@XXXX
Subject: 	Isolation and characterization of Df(3R)BSC505
Date: 	Tue, 15 Apr 2008  15:18:15  -0400  ( 20:18  BST)
Isolation and characterization of Df(3R)BSC505
Stacey Christensen, Kim Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(3R)BSC505 was isolated as a FLP recombinase-induced recombination 
event involving PBac{WH}CG1792[f06161] and P{XP}d09534. The deletion 
was isolated as a chromosome lacking miniwhite markers in progeny of 
w[1118]; Dr[1]/TM6C, Sb[1] females crossed to P{hsFLP}1, y[1] 
w[1118]; PBac{WH}CG1792[f06161]/P{XP}d09534 males. The males were 
heat shocked as larvae as described in Parks et al., Nature Genetics 
36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding 
and succeeding generations maintained the original genetic background 
of the Exelixis insertion stocks (Thibault et al., Nature Genetics 
36: 283-287, 2004; FBrf0175002). The recombination event generated 
the genetic element P+PBac{XP5.WH5}BSC505 from the segment of 
PBac{WH}CG1792[f06161] to the left of its FRT site and the segment of 
P{XP}d09534 to the right of its FRT site. Its presence was verified 
using the PCR methods and primers described in Parks et al. Exelixis, 
Inc. determined the insertion site of PBac{WH}CG1792[f06161] to be 
Release 3 genomic coordinate 27432828 on chromosome arm 3R and the 
insertion site of P{XP}d09534 to be Release 3 genomic coordinate 
27561025 on chromosome arm 3R. The Gene Disruption project determined 
the insertion site of PBac{WH}CG1792[f06161] to be Release 3 genomic 
coordinate 27432863 on arm 3R. The PBac{WH}CG1792[f06161] insertion 
site corresponds to 100C1 on the Release 3 genome map and 100D1 on 
the Release 5 genome map.  The P{XP}d09534 insertion site corresponds 
to 100C3 on the Release 3 genome map and 100D2 on the Release 5 
genome map. Consequently, the cytological breakpoints of Df(3R)BSC505 
are predicted to be 100D1;100D2. Df(3R)BSC505 failed to complement ttk[1e11].
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    English
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    Aberrations (1)
    Alleles (1)
    Genes (1)
    Insertions (3)
    Transgenic Constructs (1)