Isolation and characterization of Df(2R)BSC482
Stacey Christensen, Kim Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(2R)BSC482 was isolated as a FLP recombinase-induced recombination event involving P{XP}l(2)k05713d11115 and PBac{WH}Strn-Mlckf04333. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of P{hsFLP}1, y1 w1118; P{XP}l(2)k05713d11115/PBac{WH}Strn-Mlckf04333 males crossed to w1118; P{hs-hid}2, wgSp-1/CyO females. These males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC482 from the segment of P{XP}l(2)k05713d11115 to the left of its FRT site and the segment of PBac{WH}Strn-Mlckf04333 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. The cytological breakpoints of Df(2R)BSC482 predicted from the Release 5 genomic coordinates of the transposable element insertions sites are 52C8;52D5. Df(2R)BSC482 failed to complement Df(2R)BSC308 and Df(2R)Exel7138.