Isolation and characterization of Df(3R)BSC508
Stacey Christensen, Kim Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(3R)BSC508 was isolated as a FLP recombinase-induced recombination event involving P{XP}d06433 and PBac{WH}f06979. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of w1118; Dr1/TM6C, Sb1 females crossed to P{hsFLP}1, y1 w1118; P{XP}d06433/PBac{WH}f06979 males. The males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC508 from the segment of P{XP}d06433 to the left of its FRT site and the segment of PBac{WH}f06979 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. Exelixis, Inc. determined the insertion site of P{XP}d06433 to be Release 3 genomic coordinate 16886342 on chromosome arm 3R. This corresponds to 93B8 on the Release 3 and Release 5 genome maps. The predicted position of PBac{WH}f06979 on the Release 5 map is 93C1. Consequently, the cytological breakpoints of Df(3R)BSC508 are predicted to be 93B8;93C1. Df(3R)BSC508 failed to complement rtet07086, ppan02231 and slmb00295.