Isolation and characterization of Df(3R)BSC517
Stacey Christensen, Kim Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(3R)BSC517 was isolated as a FLP recombinase-induced recombination event involving PBac{WH}tremf05981 and P{XP}d04966. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of w1118; Dr1/TM6C, Sb1 females crossed to P{hsFLP}1, y1 w1118; PBac{WH}tremf05981/P{XP}d04966 males. The males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC517 from the segment of PBac{WH}tremf05981 to the left of its FRT site and the segment of P{XP}d04966 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. The cytological breakpoints of Df(3R)BSC517 predicted from the Release 5 genomic coordinates of the transposable element insertions sites are 92C1;92F13. Df(3R)BSC517 failed to complement Stat92E06346, and Df(3R)BSC517 heterozygotes show a Hairless (H) phenotype.