Reference Report

Reference
Citation	Pielage, J., Cheng, L., Fetter, R.D., Carlton, P.M., Sedat, J.W., Davis, G.W. (2008). A presynaptic giant ankyrin stabilizes the NMJ through regulation of presynaptic microtubules and transsynaptic cell adhesion. Neuron 58(2): 195--209. (Export to RIS)
FlyBase ID	FBrf0204637
Publication Type	Research paper
PubMed ID	18439405
PubMed Abstract	In a forward genetic screen for mutations that destabilize the neuromuscular junction, we identified a novel long isoform of Drosophila ankyrin2 (ank2-L). We demonstrate that loss of presynaptic Ank2-L not only causes synapse disassembly and retraction but also disrupts neuronal excitability and NMJ morphology. We provide genetic evidence that ank2-L is necessary to generate the membrane constrictions that normally separate individual synaptic boutons and is necessary to achieve the normal spacing of subsynaptic protein domains, including the normal organization of synaptic cell adhesion molecules. Mechanistically, synapse organization is correlated with a lattice-like organization of Ank2-L, visualized using extended high-resolution structured-illumination microscopy. The stabilizing functions of Ank2-L can be mapped to the extended C-terminal domain that we demonstrate can directly bind and organize synaptic microtubules. We propose that a presynaptic Ank2-L lattice links synaptic membrane proteins and spectrin to the underlying microtubule cytoskeleton to organize and stabilize the presynaptic terminal.
DOI	10.1016/j.neuron.2008.02.017
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Language of Publication	English
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Parent Publication
Publication Type	Journal
Abbreviation	Neuron
Title	Neuron
Publication Year	1988-
ISBN/ISSN	0896-6273
Data from Reference
Aberrations (1)
	Df(3L)RM5-2
Alleles (12)
	Ank2^{dsRNA.L.Scer\UAS} Ank2^f00518 Ank2^f02001 Ank2^{L4.Scer\UAS.T:Avic\GFP-YFP.Venus} Ank2^{L8.Scer\UAS.T:Avic\GFP-YFP.Venus} Ank2^{S.Scer\UAS.T:Avic\GFP-YFP.Venus} Scer\GAL4^C57 Scer\GAL4^elav-C155 Scer\GAL4^sca-537.4 T:Avic\GFP^YFP.Venus α-Spec^{dsRNA.Scer\UAS} β-Spec^{dsRNA.Scer\UAS}
Constructs (6)
	P{UAS-Ank2-L.RNAi} P{UAS-YFP-Ank2-L4} P{UAS-YFP-Ank2-L8} P{UAS-YFP-Ank2-S} P{UAS-α-Spec.dsRNA} P{UAS-β-Spec.dsRNA}
Genes (9)
	Ank2 brp dlg1 Fas2 futsch Scer\GAL4 Syn α-Spec β-Spec
Insertions (5)
	P{GawB}C57 P{GawB}elav^C155 P{GawB}sca^537.4 PBac{WH}Ank2^f00518 PBac{WH}Ank2^f02001
Natural transposons (1)
	P-element