Reference Report

Reference
Citation	Kaplow, M.E., Korayem, A.H., Venkatesh, T.R. (2008). Regulation of Glia Number in Drosophila by Rap/Fzr, an Activator of the Anaphase-Promoting Complex, and Loco, an RGS Protein. Genetics 178(4): 2003--2016. (Export to RIS)
FlyBase ID	FBrf0204641
Publication Type	Research paper
PubMed ID	18430931
PubMed Abstract	Glia mediate a vast array of cellular processes and are critical for nervous system development and function. Despite their immense importance in neurobiology, glia remain understudied and the molecular mechanisms that direct their differentiation are poorly understood. Rap/Fzr is the Drosophila homolog of the mammalian Cdh1, a regulatory subunit of the anaphase-promoting complex/cyclosome (APC/C). APC/C is an E3 ubiquitin ligase complex well characterized for its role in cell cycle progression. In this study, we have uncovered a novel cellular role for Rap/Fzr. Loss of rap/fzr function leads to a marked increase in the number of glia in the nervous system of third instar larvae. Conversely, ectopic expression of UAS-rap/fzr, driven by repo-GAL4, results in the drastic reduction of glia. Data from clonal analyses using the MARCM technique show that Rap/Fzr regulates the differentiation of surface glia in the developing larval nervous system. Our genetic and biochemical data further indicate that Rap/Fzr regulates glial differentiation through its interaction with Loco, a regulator of G-protein signaling (RGS) protein and a known effector of glia specification. We propose that Rap/Fzr targets Loco for ubiquitination, thereby regulating glial differentiation in the developing nervous system.
DOI	10.1534/genetics.107.086397
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Genetics
Title	Genetics
Publication Year	1916-
ISBN/ISSN	0016-6731
Data from Reference
Alleles (11)
	Ecol\lacZ^loco-rC56 Ecol\lacZ^P2241 Ecol\lacZ^rap-G0326 loco^P452 loco^{Scer\UAS.T:Avic\GFP-EGFP} rap³ rap^8F3 rap^e6 rap^G0326 rap^Scer\UAS.cKa Scer\GAL4^repo
Constructs (2)
	P{UAS-loco.GFP} P{UAS-rap.K}
Genes (8)
	dac Ecol\lacZ His3 loco mira rap repo Scer\GAL4
Insertions (4)
	P{GAL4}repo P{lacW}rap^G0326 P{lacZ-un1}loco^rC56 P{unk}rap^P2241