Reference Report

Reference
Citation	Morton, D.B., Stewart, J.A., Langlais, K.K., Clemens-Grisham, R.A., Vermehren, A. (2008). Synaptic transmission in neurons that express the Drosophila atypical soluble guanylyl cyclases, Gyc-89Da and Gyc-89Db, is necessary for the successful completion of larval and adult ecdysis. J. Exp. Biol. 211(10): 1645--1656. (Export to RIS)
FlyBase ID	FBrf0204697
Publication Type	Research paper
PubMed ID	18456892
PubMed Abstract	Insect ecdysis is a precisely coordinated series of behavioral and hormonal events that occur at the end of each molt. A great deal is known about the hormonal events that underlie this process, although less is known about the neuronal circuitry involved. In this study we identified two populations of neurons that are required for larval and adult ecdyses in the fruit fly, Drosophila melanogaster (Meigen). These neurons were identified by using the upstream region of two genes that code for atypical soluble guanylyl cyclases to drive tetanus toxin in the neurons that express these cyclases to block their synaptic activity. Expression of tetanus toxin in neurons that express Gyc-89Da blocked adult eclosion whereas expression of tetanus toxin in neurons that express Gyc-89Db prevented the initiation of the first larval ecdysis. Expression of tetanus toxin in the Gyc-89Da neurons also resulted in about 50% lethality just prior to pupariation; however, this was probably due to suffocation in the food as lethality was prevented by stopping the larvae from burrowing deep within the food. This result is consistent with our model that the atypical soluble guanylyl cyclases can act as molecular oxygen detectors. The expression pattern of these cyclases did not overlap with any of the neurons containing peptides known to regulate ecdysis and eclosion behaviors. By using the conditional expression of tetanus toxin we were also able to demonstrate that synaptic activity in the Gyc-89Da and Gyc-89Db neurons is required during early adult development for adult eclosion.
DOI	10.1242/jeb.014472
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Language of Publication	English
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Publication Type	Journal
Abbreviation	J. Exp. Biol.
Title	Journal of Experimental Biology
Publication Year	1930-
ISBN/ISSN	0022-0949
Data from Reference
Alleles (12)
	Avic\GFP^Gyc-89Da.1.9 Avic\GFP^Gyc-89Db.3.4 Btau\PDE5A^Scer\UAS.cBa Ctet\TeTxLC^{IMPTNT-V.Scer\UAS} Ctet\TeTxLC^TNT.Scer\UAS Scer\GAL4^ap-md544 Scer\GAL4^Ccap.PP Scer\GAL4^dimm-929 Scer\GAL4^Eh.2.4 Scer\GAL4^Gyc-89Da.1.9 Scer\GAL4^Gyc-89Db.3.4 Scer\GAL80^ts.αTub84B
Constructs (10)
	P{Ccap-GAL4.P} P{GAL4-Eh.2.4} P{Gyc-89Da-GAL4.1.9} P{Gyc-89Da-GFP.1.9} P{Gyc-89Db-GAL4.3.4} P{Gyc-89Db-GFP.3.4} P{tubP-GAL80^ts} P{UAS-PDE5A.B} P{UAS-TeTxLC.(-)V} P{UAS-TeTxLC.tnt}
Genes (11)
	ap Avic\GFP Btau\PDE5A Ccap Ctet\TeTxLC dimm Eh Gyc-89Da Gyc-89Db Scer\GAL4 Scer\GAL80
Insertions (2)
	P{GawB}ap^md544 P{GawB}dimm⁹²⁹
Natural transposons (1)
	P-element