Reference Report

Reference
Citation	Christensen, S., Cook, K., Cook, K. (2008.4.15). Isolation and characterization of Df(2R)BSC429. (Export to RIS)
FlyBase ID	FBrf0204795
Publication Type	Personal communication to FlyBase
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PubMed Abstract
Text of Personal Communication	From: Kevin Cook <kcook@XXXXXXXXXXXXXXX> To: flybase-updates@XXXXXXXXXXXXXXX Cc: Kim Cook <ruacookXXXXXXXXXXXXXXX>, Stacey Christensen <sjchristXXXXXXXXXXXXXXX> Subject: Isolation and characterization of Df(2R)BSC429 Date: Tue, 15 Apr 2008 14:28:24 -0400 (19:28 BST) Isolation and characterization of Df(2R)BSC429 Stacey Christensen, Kim Cook and Kevin Cook Bloomington Stock Center Indiana University Df(2R)BSC429 was isolated as a FLP recombinase-induced recombination event involving PBac{RB}CR30478[e00165] and P{XP}d03333. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of P{hsFLP}1, y[1] w[1118]; PBac{RB}CR30478[e00165]/P{XP}d03333 males crossed to w[1118]; P{hs-hid}2, wg[Sp-1]/CyO females. These males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.RB3}BSC429 from the segment of PBac{RB}CR30478[e00165] to the left of its FRT site and the segment of P{XP}d03333 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. with the substitution of the primer 5'-CCAATGCGTTTATTTCAGGTCACG-3' for the RB3' plus or RB3' minus primer in the Hybrid PCR protocol in the Supplementary Methods. The cytological breakpoints of Df(2R)BSC429 predicted from the Release 5 genomic coordinates of the transposable element insertions sites are 51C2;51D1. It failed to complement kn[1] and SMC2[jsl2]. __________________________________________________________ Kevin Cook, Ph.D. Bloomington Drosophila Stock Center Department of Biology http://flystocks.bio.indiana.edu Jordan Hall 142 Indiana University 812-856-1213 1001 E. Third St. 812-855-2577 (fax) Bloomington, IN 47405-7005 kcook@XXXXXXXXXXXXXXX
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Language of Publication	English
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Data from Reference
Aberrations (1)
	Df(2R)BSC429
Alleles (2)
	kn¹ SMC2^jsl2
Constructs (2)
	P+PBac{XP5.RB3} PBac{RB}
Genes (2)
	kn SMC2
Insertions (3)
	P{XP}d03333 P+PBac{XP5.RB3}BSC429 PBac{RB}CR30478^e00165