Reference Report

Reference
Citation	Rudolph, T., Yonezawa, M., Lein, S., Heidrich, K., Kubicek, S., Schafer, C., Phalke, S., Walther, M., Schmidt, A., Jenuwin, T., Reuter, G. (2007). Heterochromatin Formation in Drosophila Is Initiated through Active Removal of H3K4 Methylation by the LSD1 Homolog SU(VAR)3-3. Mol. Cell 26(1): 103--115. (Export to RIS)
FlyBase ID	FBrf0204799
Publication Type	Research paper
PubMed ID	17434130
PubMed Abstract	Epigenetic indexing of chromatin domains by histone lysine methylation requires the balanced coordination of methyltransferase and demethylase activities. Here, we show that SU(VAR)3-3, the Drosophila homolog of the human LSD1 amine oxidase, demethylates H3K4me2 and H3K4me1 and facilitates subsequent H3K9 methylation by SU(VAR)3-9. Su(var)3-3 mutations suppress heterochromatic gene silencing, display elevated levels of H3K4me2, and prevent extension of H3K9me2 at pericentric heterochromatin. SU(VAR)3-3 colocalizes with H3K4me2 in interband regions and is abundant during embryogenesis and in syncytial blastoderm, where it appears concentrated at prospective heterochromatin during cycle 14. In embryos of Su(var)3-3/+ females, H3K4me2 accumulates in primordial germ cells, and the deregulated expansion of H3K4me2 antagonizes heterochromatic H3K9me2 in blastoderm cells. Our data indicate an early developmental function for the SU(VAR)3-3 demethylase in controlling euchromatic and heterochromatic domains and reveal a hierarchy in which SU(VAR)3-3-mediated removal of activating histone marks is a prerequisite for subsequent heterochromatin formation by H3K9 methylation.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Mol. Cell
Title	Molecular Cell
Publication Year	1997-
ISBN/ISSN	1097-2765 1097-4164
Data from Reference
Aberrations (5)
	Dp(1;f)γ238 In(1)w^m4 In(2R)bw^VDe1 In(3L)BL1 T(2;3)Sb^V
Alleles (16)
	Su(var)3-3¹ Su(var)3-3² Su(var)3-3⁵ Su(var)3-3⁶ Su(var)3-3⁷ Su(var)3-3⁸ Su(var)3-3⁹ Su(var)3-3¹⁰ Su(var)3-3¹¹ Su(var)3-3¹² Su(var)3-3¹³ Su(var)3-3¹⁴ Su(var)3-3¹⁵ Su(var)3-3²¹ Su(var)3-3^f00678 Su(var)3-3^unspecified
Genes (4)
	CG17147 His3 Su(var)3-3 Su(var)3-9
Insertions (1)
	PBac{WH}Su(var)3-3^f00678