Reference Report

Reference
Citation	Bachmann, A., Grawe, F., Johnson, K., Knust, E. (2008). Drosophila Lin-7 is a component of the Crumbs complex in epithelia and photoreceptor cells and prevents light-induced retinal degeneration. Europ. J. Cell Biol. 87(3): 123--136. (Export to RIS)
FlyBase ID	FBrf0204878
Publication Type	Research paper
PubMed ID	18177979
PubMed Abstract	The Drosophila Crumbs protein complex is required to maintain epithelial cell polarity in the embryo, to ensure proper morphogenesis of photoreceptor cells and to prevent light-dependent retinal degeneration. In Drosophila, the core components of the complex are the transmembrane protein Crumbs, the membrane-associated guanylate kinase (MAGUK) Stardust and the scaffolding protein DPATJ. The composition of the complex and some of its functions are conserved in mammalian epithelial and photoreceptor cells. Here, we report that Drosophila Lin-7, a scaffolding protein with one Lin-2/Lin-7 (L27) domain and one PSD-95/Dlg/ZO-1 (PDZ) domain, is associated with the Crumbs complex in the subapical region of embryonic and follicle epithelia and at the stalk membrane of adult photoreceptor cells. DLin-7 loss-of-function mutants are viable and fertile. While DLin-7 localization depends on Crumbs, neither Crumbs, Stardust nor DPATJ require DLin-7 for proper accumulation in the subapical region. Unlike other components of the Crumbs complex, DLin-7 is also enriched in the first optic ganglion, the lamina, where it co-localizes with Discs large, another member of the MAGUK family. In contrast to crumbs mutant photoreceptor cells, those mutant for DLin-7 do not display any morphogenetic abnormalities. Similar to crumbs mutant eyes, however, DLin-7 mutant photoreceptors undergo progressive, light-dependent degeneration. These results support the previous conclusions that the function of the Crumbs complex in cell survival is independent from its function in photoreceptor morphogenesis.
DOI	10.1016/j.ejcb.2007.11.002
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Europ. J. Cell Biol.
Title	European Journal of Cell Biology
Publication Year	1979-
ISBN/ISSN	0171-9335
Data from Reference
Alleles (7)
	crb^11A22 crb^{intra.Scer\UAS.T:Hsap\MYC} Scer\GAL4^en-e16E veli⁵⁴ veli⁶⁶ veli^GE20172 veli^{Scer\UAS.T:Zzzz\FLAG}
Constructs (4)
	P{?EP} P{EP} P{UAS-crb.myc} P{UAS-veli.FLAG}
Genes (6)
	crb dlg1 Patj Scer\GAL4 sdt veli
Insertions (3)
	P{?EP}veli⁵⁴ P{en2.4-GAL4}e16E P{EP}veli^GE20172