Reference Report

Reference
Citation	Kadener, S., Menet, J.S., Schoer, R., Rosbash, M. (2008). Circadian transcription contributes to core period determination in Drosophila. PLoS Biol. 6(5): e119. (Export to RIS)
FlyBase ID	FBrf0204901
Publication Type	Research paper
PubMed ID	18494558
PubMed Abstract	The Clock-Cycle (CLK-CYC) heterodimer constitutes a key circadian transcription complex in Drosophila. CYC has a DNA-binding domain but lacks an activation domain. Previous experiments also indicate that most of the transcriptional activity of CLK-CYC derives from the glutamine-rich region of its partner CLK. To address the role of transcription in core circadian timekeeping, we have analyzed the effects of a CYC-viral protein 16 (VP16) fusion protein in the Drosophila system. The addition of this potent and well-studied viral transcriptional activator (VP16) to CYC imparts to the CLK-CYC-VP16 complex strongly enhanced transcriptional activity relative to that of CLK-CYC. This increase is manifested in flies expressing CYC-VP16 as well as in S2 cells. These flies also have increased levels of CLK-CYC direct target gene mRNAs as well as a short period, implicating circadian transcription in period determination. A more detailed examination of reporter gene expression in CYC-VP16-expressing flies suggests that the short period is due at least in part to a more rapid transcriptional phase. Importantly, the behavioral effects require a period (per) promoter and are therefore unlikely to be merely a consequence of generally higher PER levels. This indicates that the CLK-CYC-VP16 behavioral effects are a consequence of increased per transcription. All of this also suggests that the timing of transcriptional activation and not the activation itself is the key event responsible for the behavioral effects observed in CYC-VP16-expressing flies. The results taken together indicate that circadian transcription contributes to core circadian function in Drosophila.
DOI	10.1371/journal.pbio.0060119
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Language of Publication	English
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Publication Type	Journal
Abbreviation	PLoS Biol.
Title	PLoS Biology
Publication Year	2003-
ISBN/ISSN	1545-7885 1544-9173
Data from Reference
Alleles (12)
	Clk^ar Clk^T:SV5\V5 cyc^{Scer\UAS.T:Hsim\VP16} cyc^{Scer\UAS.T:Ivir\HA1} per⁰¹ per^s per^Scer\UAS.cYa Scer\GAL4^Act.PU Scer\GAL4^elav-C155 Scer\GAL4^P2.4.Pdf Scer\GAL4^tim.PE w^+mC
Constructs (7)
	P{Act-GAL4.U} P{Clk.V5} P{GAL4-tim.E} P{Pdf-GAL4.P2.4} P{UAS-CYC.HA} P{UAS-cyc.VP16} P{UAS-per.Y}
Genes (13)
	CG9649 Clk cry cyc Pdp1 per Scer\GAL4 T:Hsim\VP16 T:SV5\V5 tim Ugt35b vri w
Insertions (1)
	P{GawB}elav^C155