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Citation
Ceprani, F., Raffa, G.D., Petrucci, R., Piergentili, R. (2008). Autosomal mutations affecting Y chromosome loops in Drosophila melanogaster.  BMC Genet. 9(): 32.
FlyBase ID
FBrf0204958
Publication Type
Research paper
Abstract

The Y chromosome of Drosophila melanogaster harbors several genes required for male fertility. The genes for these fertility factors are very large in size and contain conspicuous amounts of repetitive DNA and transposons. Three of these loci (ks-1, kl-3 and kl-5) have the ability to develop giant lampbrush-like loops in primary spermatocytes, a cytological manifestation of their active state in these cells. Y-loops bind a number of non-Y encoded proteins, but the mechanisms regulating their development and their specific functions are still to be elucidated.Here we report the results of a screen of 726 male sterile lines to identify novel autosomal genes controlling Y-loop function. We analyzed mutant testis preparations both in vivo and by immunofluorescence using antibodies directed against Y-loop-associated proteins. This screen enabled us to isolate 17 mutations at 15 loci whose wild-type function is required for proper Y-loop morphogenesis. Six of these loci are likely to specifically control loop development, while the others display pleiotropic effects on both loops and meiotic processes such as spermiogenesis, sperm development and maturation. We also determined the map position of the mutations affecting exclusively Y-loop morphology.Our cytological screening permitted us to identify novel genetic functions required for male spermatogenesis, some of which show pleiotropic effects. Analysis of these mutations also shows that loop development can be uncoupled from meiosis progression. These data represent a useful framework for the characterization of Y-loop development at a molecular level and for the study of the genetic control of heterochromatin.

PubMed ID
PubMed Central ID
PMC2386818 (PMC) (EuropePMC)
Related Publication(s)
Personal communication to FlyBase

Symbols for male sterile mutations affecting Y chromosome loops.
Piergentili, 2008.5.28, Symbols for male sterile mutations affecting Y chromosome loops. [FBrf0204993]

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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    BMC Genet.
    Title
    BMC Genetics
    Publication Year
    2000-
    ISBN/ISSN
    1471-2156
    Data From Reference