Isolation and characterization of Df(3R)BSC524
Stacey Christensen, Kim Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(3R)BSC524 was isolated as a FLP recombinase-induced recombination event involving PBac{WH}f06726 and P{XP}d05561. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of w1118; P{hs-hid}3, Dr1/TM6C, Sb1 females crossed to P{hsFLP}1, y1 w1118; PBac{WH}f06726/P{XP}d05561 males. The males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC524 from the segment of PBac{WH}f06726 to the left of its FRT site and the segment of P{XP}d05561 to the right of its FRT site. Exelixis, Inc. determined the insertion site of P{XP}d05561 to be Release 3 genomic coordinate 22773773 on chromosome arm 3R. This corresponds to 97D11 on the Release 3 and Release 5 genome maps. The predicted position of PBac{WH}f06726 on the Release 5 map is 97C3. Consequently, the cytological breakpoints of Df(3R)BSC524 are predicted to be 97C3;97D11. Df(3R)BSC524 failed to complement His2Av810 and Rb97D1.