Isolation and characterization of Df(1)BSC532
Stacey Christensen, Kim Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(1)BSC532 was isolated as a FLP recombinase-induced recombination event involving P{XP}norpAd07261 and PBac{WH}f07985. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of P{XP}norpAd07261/PBac{WH}f07985; MKRS, P{hsFLP}86E/+ females crossed to Binsinscy/Y males. These females were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC532 from the segment of P{XP}norpAd07261 to the left of its FRT site and the segment of PBac{WH}f07985 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. Exelixis, Inc. determined the insertion site of PBac{WH}f07985 to be Release 3 genomic coordinate 4549729 on the X chromosome. This corresponds to 4D2 on the Release 3 and Release 5 genome maps. The predicted position of P{XP}norpAd07261 on the Release 5 map is 4C1. Consequently, the cytological breakpoints of Df(1)BSC532 are predicted to be 4C1;4D2. It failed to complement rb1, bi1 and peb1.