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Reference Report
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| Citation | Christensen, S., Cook, K., Cook, K. (2008.7.11). Isolation and characterization of Df(3L)BSC556. (Export to RIS) | ||
| FlyBase ID | FBrf0205001 | ||
| Publication Type | Personal communication to FlyBase | ||
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| Text of Personal Communication |
From: Kevin Cook <kcook@XXXXXXXXXXXXXXX>
Subject: Isolation and characterization of Df(3L)BSC556 Date: Fri, 11 Jul 2008 13:19:29 -0400 (18:19 BST) Isolation and characterization of Df(3L)BSC556 Stacey Christensen, Kim Cook and Kevin Cook Bloomington Stock Center Indiana University Df(3L)BSC556 was isolated as a FLP recombinase-induced recombination event involving P{XP}ImpL2[d01988] and PBac{RB}CG32249[e02744]. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of w[1118]; P{hs-hid}3, Dr[1]/TM6C, Sb[1] females crossed to P{hsFLP}1, y[1] w[1118]; P{XP}ImpL2[d01988]/PBac{RB}CG32249[e02744] males. The males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.RB3}BSC556 from the segment of P{XP}ImpL2[d01988] to the left of its FRT site and the segment of PBac{RB}CG32249[e02744] to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. with the substitution of the primer 5'-CCAATGCGTTTATTTCAGGTCACG-3' for the RB3' plus or RB3' minus primer in the Hybrid PCR protocol in the Supplementary Methods. The cytological breakpoints of Df(3L)BSC556 predicted from the Release 5 genomic coordinates of the transposable element insertions sites are 64A10;64B7. Df(3L)BSC556 failed to complement pav[B200] and Df(3L)Exel8098. __________________________________________________________ Kevin Cook, Ph.D. Bloomington Drosophila Stock Center Department of Biology http://flystocks.bio.indiana.edu Jordan Hall 142 Indiana University 812-856-1213 1001 E. Third St. 812-855-2577 (fax) Bloomington, IN 47405-7005 kcook@XXXXXXXXXXXXXXX |
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| Language of Publication | English | ||
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Data from Reference
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| Aberrations (2)
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| Alleles (1)
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| Constructs (1)
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| Genes (1)
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| Insertions (3)
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