Reference Report

Reference
Citation	Christensen, S., Cook, K., Cook, K. (2008.7.11). Isolation and characterization of Df(3L)BSC562. (Export to RIS)
FlyBase ID	FBrf0205007
Publication Type	Personal communication to FlyBase
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PubMed Abstract
Text of Personal Communication	From: Kevin Cook <kcook@XXXXXXXXXXXXXXX> Subject: Isolation and characterization of Df(3L)BSC562 Date: Fri, 11 Jul 2008 13:22:29 -0400 (18:22 BST) Isolation and characterization of Df(3L)BSC562 Stacey Christensen, Kim Cook and Kevin Cook Bloomington Stock Center Indiana University Df(3L)BSC562 was isolated as a FLP recombinase-induced recombination event involving P{XP}d07163 and PBac{RB}tra[e01760]. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of w[1118]; P{hs-hid}3, Dr[1]/TM6C, Sb[1] females crossed to P{hsFLP}1, y[1] w[1118]; P{XP}d07163/PBac{RB}tra[e01760] males. The males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.RB3}BSC562 from the segment of P{XP}d07163 to the left of its FRT site and the segment of PBac{RB}tra[e01760] to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. with the substitution of the primer 5'-CCAATGCGTTTATTTCAGGTCACG-3' for the RB3' plus or RB3' minus primer in the Hybrid PCR protocol in the Supplementary Methods. The cytological breakpoints of Df(3L)BSC562 predicted from the Release 5 genomic coordinates of the transposable element insertions sites are 73A1;73A10. Df(3L)BSC562 failed to complement argos[W11] and st[sp]. __________________________________________________________ Kevin Cook, Ph.D. Bloomington Drosophila Stock Center Department of Biology http://flystocks.bio.indiana.edu Jordan Hall 142 Indiana University 812-856-1213 1001 E. Third St. 812-855-2577 (fax) Bloomington, IN 47405-7005 kcook@XXXXXXXXXXXXXXX
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Language of Publication	English
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Data from Reference
Aberrations (1)
	Df(3L)BSC562
Alleles (2)
	aos^W11 st^sp
Constructs (1)
	P+PBac{XP5.RB3}
Genes (2)
	aos st
Insertions (3)
	P{XP}CG33158^d07163 P+PBac{XP5.RB3}BSC562 PBac{RB}tra^e01760